Poly(hydroxyethyl methacrylate) membranes: As a hydrogel support for use in immobilized metal affinity chromatography
Yükleniyor...
Tarih
2003
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Lysozyme adsorption onto a dye ligand (Procion Red HE-3B) immobilized and Cu(II) incorporated poly(2-hydroxyethylmethacrylate) (pHEMA) membrane were investigated. The membranes were prepared by UV initiated photopolymerization of HEMA in the presence of an initiator (alpha-alpha'-azoisobutyronitrile; AIBN). The amount of immobilized dye on the membrane was 112.2 mumol g(-1). Lysozyme adsorption on to these membranes from aqueous solutions containing different amounts of lysozyme at different pH was investigated in batch system. Lysozyme adsorption capacity of the dye-ligand immobilized membrane was 45.6 mg g(-1). Lysozyme adsorption capacity of the Cu(II) incorporated membranes (112.3 mg g(-1)) was greater than that of the Procion Red HE-3B immobilized membranes. The non-specific adsorption of lysozyme on the pHEMA membranes was 0.14 mg g(-1). More than 97% of the adsorbed lysozyme were desorbed in 60 in the desorption medium containing 1.0 M KSCN at pH 8.0.
Açıklama
4th International Conference on Polymer Solvent Complexes and Intercalates -- JUL 22-25, 2002 -- PRAGUE, CZECH REPUBLIC
Anahtar Kelimeler
adsorption, hydrophilic polymer, lysozyme, membranes, procion red HE-3B
Kaynak
Macromolecular Symposia
WoS Q Değeri
Q2
Scopus Q Değeri
Q3
Cilt
203
Sayı
Künye
closedAccess
