Poly(hydroxyethyl methacrylate) membranes: As a hydrogel support for use in immobilized metal affinity chromatography

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dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorArıca, M. Yakup
dc.date.accessioned2020-06-25T17:35:18Z
dc.date.available2020-06-25T17:35:18Z
dc.date.issued2003
dc.departmentKırıkkale Üniversitesi
dc.description4th International Conference on Polymer Solvent Complexes and Intercalates -- JUL 22-25, 2002 -- PRAGUE, CZECH REPUBLIC
dc.description.abstractLysozyme adsorption onto a dye ligand (Procion Red HE-3B) immobilized and Cu(II) incorporated poly(2-hydroxyethylmethacrylate) (pHEMA) membrane were investigated. The membranes were prepared by UV initiated photopolymerization of HEMA in the presence of an initiator (alpha-alpha'-azoisobutyronitrile; AIBN). The amount of immobilized dye on the membrane was 112.2 mumol g(-1). Lysozyme adsorption on to these membranes from aqueous solutions containing different amounts of lysozyme at different pH was investigated in batch system. Lysozyme adsorption capacity of the dye-ligand immobilized membrane was 45.6 mg g(-1). Lysozyme adsorption capacity of the Cu(II) incorporated membranes (112.3 mg g(-1)) was greater than that of the Procion Red HE-3B immobilized membranes. The non-specific adsorption of lysozyme on the pHEMA membranes was 0.14 mg g(-1). More than 97% of the adsorbed lysozyme were desorbed in 60 in the desorption medium containing 1.0 M KSCN at pH 8.0.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/masy.200351320
dc.identifier.endpage212en_US
dc.identifier.issn1022-1360
dc.identifier.scopus2-s2.0-0347480291
dc.identifier.scopusqualityQ3
dc.identifier.startpage207en_US
dc.identifier.urihttps://doi.org/10.1002/masy.200351320
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3082
dc.identifier.volume203en_US
dc.identifier.wosWOS:000187285200021
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofMacromolecular Symposia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectadsorptionen_US
dc.subjecthydrophilic polymeren_US
dc.subjectlysozymeen_US
dc.subjectmembranesen_US
dc.subjectprocion red HE-3Ben_US
dc.titlePoly(hydroxyethyl methacrylate) membranes: As a hydrogel support for use in immobilized metal affinity chromatographyen_US
dc.typeArticle

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