The apoptotic and proliferative effects of tulathromycin and gamithromycin on bovine tracheal epithelial cell culture

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dc.contributor.authorSahin, Yasar
dc.contributor.authorYildirim, Ebru
dc.contributor.authorYurdakok Dikmen, Begum
dc.contributor.authorTurk, Mustafa
dc.date.accessioned2025-01-21T16:34:50Z
dc.date.available2025-01-21T16:34:50Z
dc.date.issued2022
dc.departmentKırıkkale Üniversitesi
dc.description.abstractGamithromycin and tulathromycin are commonly used in the treatment of bovine respiratory bacterial diseases. The current work was undertaken to establish the apoptotic, necrotic, and cytotoxic effects of these antibiotics in the target animal. Cells with apoptosis and necrosis were determined by dual staining method, cytotoxic effects were determined by MTT assay, cell proliferative effects were examined by XCelligence real-time cell analysis system (RTCA-SP). The comparison between gamithromycin and tulathromycin concentrations on tracheal cells in terms of % cell viability was found to be significantly different. While the cell viability percentage of gamithromycin was higher at 150 mu g/mL, 180 mu g/mL, and 240 mu g/mL than tulathromycin, and at 2 mu g/mL, 4 mu g/mL, 10 mu g/mL, 20 mu g/mL, and 50 mu g/mL concentrations tulathromycin cell viability was higher than gamithromycin (p < 0.05). When the staining method data were evaluated, the difference between the results of % apoptotic index at 20 mu g/mL concentration was significant and it was found that gamithromycin had more apoptotic effect than tulathromycin (p < 0.05). It was seen that tulathromycin and gamithromycin applied on tracheal epithelial cells at concentrations of 2 and 10 mu g/mL increased the viability depending on time. The increase in epithelial cell proliferation of gamithromycin and tulathromycin due to time shows that these antibiotics can maintain longterm prophylactic treatment against diseases.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Kirikkale University [2018/047]
dc.description.sponsorshipThis study summarized from a part of the thesis titled The investigation of the effects of tulathromycin and gamithromycin on tracheal smooth muscle contraction and the apoptotic, necrotic and cytotoxic effects on tracheal epithelial cells in bovine. This work was supported by the Scientific Research Projects Coordination Unit of Kirikkale University with the project number 2018/047.
dc.identifier.doi10.3906/vet-2107-45
dc.identifier.endpage146
dc.identifier.issn1300-0128
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85125185819
dc.identifier.scopusqualityQ3
dc.identifier.startpage139
dc.identifier.trdizinid527349
dc.identifier.urihttps://doi.org/10.3906/vet-2107-45
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay527349
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24006
dc.identifier.volume46
dc.identifier.wosWOS:000758128300001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Veterinary & Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.subjectBovine trachea; epithelial cell; gamithromycin; proliferation; tulathromycin
dc.titleThe apoptotic and proliferative effects of tulathromycin and gamithromycin on bovine tracheal epithelial cell culture
dc.typeArticle

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