Expression of glutathione-S-transferases isoenzymes and p53 in exfoliated human bladder cancer cells

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dc.contributor.authorOguztuzun, Serpil
dc.contributor.authorSezgin, Yasemin
dc.contributor.authorYazici, Sertac
dc.contributor.authorFirat, Pinar
dc.contributor.authorOzhavzali, Muzeyyen
dc.contributor.authorOzen, Haluk
dc.date.accessioned2020-06-25T17:51:42Z
dc.date.available2020-06-25T17:51:42Z
dc.date.issued2011
dc.departmentKırıkkale Üniversitesi
dc.description.abstractObjectives: This study investigates the usefulness of glutathione-S-transferases (GST) isoenzymes and p53 immunostaining as a marker of malignancy in urinary cytology, and evaluates their potential effect in increasing diagnostic accuracy in a series of urine cytologic samples. They are also correlated with cytopathology diagnosis and histopathologic diagnosis. Materials and methods: In this study, the slides from 124 bladder carcinoma patients prepared by the cytocentrifugation method were observed. The cytomorphologic properties of these cancer cells were determined. Moreover, the immunocytochemical distributions of GST alpha (GSTA), pi (GSTP), mu (GSTM4), theta (GSTT1) isoenzymes and p53 protein were studied for the patients. Results: The urothelial cancer cells had small cytoplasm and rough nuclear membrane. The chromatin granules were heterogeneously distributed in each malignant cell's nucleus. There was a pleomorphism of the malignant cells' nuclei. According to immunocytopathologic observations, the urothelial cancer cells had stronger staining intensity than the benign cells had in 48% of cases for GSTA, 46% of cases for GSTP, 38% of cases for GSTM4, and 42% of cases for GSTT1. For all papillary cases, the malignant cells were stained negative, while the benign cells were positive. For 83% of patients, the malignant cells were stained positive for p53. There was a significant difference in GSTA (P = 0.006), GSTT1 (P = 0.004), GSTP (P = 0.000) and p53 (P = 0.000) expressions for benign cells whereas, a non-statistical difference in the malignant cells for GSTA, GSTT1, GSTP, GSTM4, and p53 expressions (P > 0.05). Conclusions: GST isoenzymes and p53 immunostaining were not found to be markers of malignancy in urinary cytology. (C) 2011 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipKirikkale UniversityKirikkale University [02/03.04.04]en_US
dc.description.sponsorshipThis work was supported by the Research Fund of the Kirikkale University, project number: 02/03.04.04.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.urolonc.2009.08.001
dc.identifier.endpage544en_US
dc.identifier.issn1078-1439
dc.identifier.issn1873-2496
dc.identifier.issue5en_US
dc.identifier.pmid19926314
dc.identifier.scopus2-s2.0-80052930544
dc.identifier.scopusqualityQ1
dc.identifier.startpage538en_US
dc.identifier.urihttps://doi.org/10.1016/j.urolonc.2009.08.001
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4961
dc.identifier.volume29en_US
dc.identifier.wosWOS:000295609000011
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Incen_US
dc.relation.ispartofUrologic Oncology-Seminars And Original Investigations
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBladder canceren_US
dc.subjectCytologyen_US
dc.subjectImmunocytochemistryen_US
dc.subjectGlutathione-S-transferaseen_US
dc.subjectp53en_US
dc.titleExpression of glutathione-S-transferases isoenzymes and p53 in exfoliated human bladder cancer cellsen_US
dc.typeArticle

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