Morphology and quantification of sheep pineal glands at pre-pubertal, pubertal and post-pubertal periods

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dc.contributor.authorBolat, Durmus
dc.contributor.authorKueruem, Aytuel
dc.contributor.authorCanpolat, Sila
dc.date.accessioned2020-06-25T18:29:32Z
dc.date.available2020-06-25T18:29:32Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.descriptionBolat, Durmus/0000-0001-7619-1913
dc.description.abstractThe pineal gland is a neuroendocrine organ associated with photoperiodic regulation in mammals. The aim of this study was to evaluate the pineal gland at the pre-pubertal, pubertal and post-pubertal periods by means of morphology and stereology. The study examined at total of 24 ovine pineal glands collected from healthy female Akkaraman breed. Thick sections (40m) were cut and treated with synaptophysin. Following each thick section, six consecutive sections at a thickness of 5m were cut. Each thin section was stained with one of the following dyes: Crossman's modified triple dye, glial fibrillary acidic protein (GFAP), melatonin marker, periodic acid-Schiff, Von Kossa and AgNOR. The pineal gland volume was measured using Cavalieri's method. The optical fractionator was used to estimate the total number of pinealocytes. The percentage of parenchyma and connective tissue and degree of vascularization were estimated by the area fraction fractionator method. The pineal gland volumes in the pre-pubertal, pubertal and post-pubertal groups were 7.53 +/- 1.715mm(3), 11.20 +/- 1.336mm(3) and 17.75 +/- 1.188mm(3), respectively (p<.5). The number of pinealocytes in the pre-pubertal, pubertal and post-pubertal groups was 3,244,000 +/- 228,076, 4,438,000 +/- 243,610, 7,381,766 +/- 406,223, respectively (p<.05). The glands of the post-pubertal group contained the highest amount of connective tissue (11.49 +/- 2.103%; p<.5) and the largest GFAP staining area (p<.05). The melatonin staining density was the highest in the pubertal group. The density of lipofuscin staining was higher in the pubertal and post-pubertal groups.en_US
dc.description.sponsorshipTurkiye Bilimsel ve Teknolojik Arastirma KurumuTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG 1130578]en_US
dc.description.sponsorshipTurkiye Bilimsel ve Teknolojik Arastirma Kurumu, Grant/Award Number: TOVAG 1130578en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1111/ahe.12359
dc.identifier.endpage345en_US
dc.identifier.issn0340-2096
dc.identifier.issn1439-0264
dc.identifier.issue4en_US
dc.identifier.pmid29774950
dc.identifier.scopus2-s2.0-85047642490
dc.identifier.scopusqualityQ2
dc.identifier.startpage338en_US
dc.identifier.urihttps://doi.org/10.1111/ahe.12359
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7359
dc.identifier.volume47en_US
dc.identifier.wosWOS:000438337300008
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWileyen_US
dc.relation.ispartofAnatomia Histologia Embryologia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectarea fraction fractionatoren_US
dc.subjectCavalieri's methoden_US
dc.subjectGFAPen_US
dc.subjectnucleatoren_US
dc.subjectoptical fractionatoren_US
dc.subjectpineal glanden_US
dc.titleMorphology and quantification of sheep pineal glands at pre-pubertal, pubertal and post-pubertal periodsen_US
dc.typeArticle

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