Chitosan-grafted poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible enzyme immobilization
Yükleniyor...
Tarih
2007
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Epoxy group-contain g poly(hydroxyethyl methacrylate/glycidyl methacrylate), p(HEMA/GMA), membrane was prepared by UV initiated photopolymerization. The membrane was grafted with chitosan (CH) and some of them were chelated with Fe(III) ions. The CH grafted, p(HEMA/GMA), and Fe(III) ions incorporated p(HEMA/ GMA)-CH-Fe(III) membranes were used for glucose oxidase (GOD) immobilization via adsorption. The maximum enzyme immobilization capacity of the p(HEMA/GMA)-CH and p(HEMA/GMA)-CH-Fe(HI) membranes were 0.89 and 1.36 mg/mL, respectively. The optimal PH value for the immobilized GOD preparations is found to have shifted 0.5 units to more acidic PH 5.0. Optimum temperature for both immobilized preparations was 10 degrees C higher than that of the free enzyme and was significantly broader at higher temperatures. The apparent K-m values were found to be 6.9 and 5.8 mM for the adsorbed GOD on p(HEMA/GMA)-CH and p(HEMA/GMA)-CH-Fe(HI) membranes, respectively. In addition, all the membranes surfaces were characterized by contact angle measurements. (c) 2006 Wiley Periodicals, Inc.
Açıklama
Anahtar Kelimeler
membrane, contact angle, immobilized enzyme, adsorption, glucose oxidase
Kaynak
Journal Of Applied Polymer Science
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
103
Sayı
5
Künye
closedAccess
