Chitosan-grafted poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible enzyme immobilization

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dc.contributor.authorArıca, M. Yakup
dc.contributor.authorYılmaz, Meltem
dc.contributor.authorBayramoğlu, Gülay
dc.date.accessioned2020-06-25T17:43:55Z
dc.date.available2020-06-25T17:43:55Z
dc.date.issued2007
dc.description.abstractEpoxy group-contain g poly(hydroxyethyl methacrylate/glycidyl methacrylate), p(HEMA/GMA), membrane was prepared by UV initiated photopolymerization. The membrane was grafted with chitosan (CH) and some of them were chelated with Fe(III) ions. The CH grafted, p(HEMA/GMA), and Fe(III) ions incorporated p(HEMA/ GMA)-CH-Fe(III) membranes were used for glucose oxidase (GOD) immobilization via adsorption. The maximum enzyme immobilization capacity of the p(HEMA/GMA)-CH and p(HEMA/GMA)-CH-Fe(HI) membranes were 0.89 and 1.36 mg/mL, respectively. The optimal PH value for the immobilized GOD preparations is found to have shifted 0.5 units to more acidic PH 5.0. Optimum temperature for both immobilized preparations was 10 degrees C higher than that of the free enzyme and was significantly broader at higher temperatures. The apparent K-m values were found to be 6.9 and 5.8 mM for the adsorbed GOD on p(HEMA/GMA)-CH and p(HEMA/GMA)-CH-Fe(HI) membranes, respectively. In addition, all the membranes surfaces were characterized by contact angle measurements. (c) 2006 Wiley Periodicals, Inc.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/app.25466
dc.identifier.endpage3093en_US
dc.identifier.issn0021-8995
dc.identifier.issn1097-4628
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-33847421351
dc.identifier.scopusqualityQ2
dc.identifier.startpage3084en_US
dc.identifier.urihttps://doi.org10.1002/app.25466
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3946
dc.identifier.volume103en_US
dc.identifier.wosWOS:000243765100039
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmembraneen_US
dc.subjectcontact angleen_US
dc.subjectimmobilized enzymeen_US
dc.subjectadsorptionen_US
dc.subjectglucose oxidaseen_US
dc.titleChitosan-grafted poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible enzyme immobilizationen_US
dc.typeArticle

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