Testicular tissue nitric oxide and thiobarbituric acid reactive substance levels: evaluation with respect to the pathogenesis of varicocele

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dc.contributor.authorKisa, U
dc.contributor.authorBasar, MM
dc.contributor.authorFerhat, M
dc.contributor.authorYilmaz, E
dc.contributor.authorBasar, H
dc.contributor.authorCaglayan, O
dc.contributor.authorBatislam, E
dc.date.accessioned2020-06-25T17:40:07Z
dc.date.available2020-06-25T17:40:07Z
dc.date.issued2004
dc.departmentKırıkkale Üniversitesi
dc.descriptionKISA, Ucler/0000-0002-8131-6810; Batislam, Ertan/0000-0002-7493-4573
dc.description.abstractThe aim of the present study is to evaluate tissue nitric oxide (NO) and thiobarbituric acid reactive substance (TBARS) levels in testicular tissue, and to determine their relationship with seminal parameters in order to explain possible effects on varicocele pathophysiology.Ten adult male Wistar rats at 8 weeks old underwent partial left renal vein ligation. A sham operation was performed on control rats in a second group of another ten rats. All animals were killed 4 weeks after surgery. The testes were removed and histological changes were observed by light microscopy with haematoxylin and eosin stain on half of each testis. The rest of testis was used for the evaluation of testicular tissue NO and TBARS levels. Epididymal aspirated seminal plasma was used for semen analysis and morphological analysis was carried out according to Kruger's criteria. Statistical analysis was performed by using Mann-Whitney U-tests and Spearman rank correlations between the two groups for NO and TBARS levels and for seminal parameters. Testicular tissue NO and TBARS levels (mean+/-SEM) were 62.8+/-10.1 mumol/g protein and 4.7+/-0.3 nmol/g protein in group 1. These parameters were 16.9+/-2.2 mumol/g protein and 3.1+/-0.2 nmol/g protein in the group 2 controls. There were significant differences between these parameters (P-NO=0.000, P-TBARS=0.001). Although a positive and significant correlation between testicular tissue NO and TBARS levels was found (r(s)=0.739, P=0.014), there was only a strong negative correlation between NO levels and sperm motility in group 1 (r(s)=-0.815, P=0.004). We found that this effect of NO on sperm motility was independent from TBARS levels after regression analysis (r(2)=-0.687, beta=0.825, P=0.034). Although there were statistically significant differences in seminal parameters between the two groups, there was no difference between them in the histopathological examination. We found that sperm motility was significantly related to testicular tissue NO levels only. Thus, we suggest that NO is an important mediator in the pathogenesis of varicocele. TBARS and other substances have been effective via NO pathways.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1007/s00240-004-0401-2
dc.identifier.endpage199en_US
dc.identifier.issn0300-5623
dc.identifier.issn1434-0879
dc.identifier.issue3en_US
dc.identifier.pmid15205852
dc.identifier.scopus2-s2.0-3142701306
dc.identifier.scopusqualityN/A
dc.identifier.startpage196en_US
dc.identifier.urihttps://doi.org/10.1007/s00240-004-0401-2
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3294
dc.identifier.volume32en_US
dc.identifier.wosWOS:000222082700004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringeren_US
dc.relation.ispartofUrological Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectvaricoceleen_US
dc.subjectnitric oxideen_US
dc.subjectTBARSen_US
dc.subjectinfertilityen_US
dc.titleTesticular tissue nitric oxide and thiobarbituric acid reactive substance levels: evaluation with respect to the pathogenesis of varicoceleen_US
dc.typeArticle

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