Synthesis, characterization and modification of Gum Arabic microgels for hemocompatibility and antimicrobial studies

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Dosyalar

Synthesis, characterization and modification of Gum Arabic microgels for hemocompatibility and antimicrobial studies.pdf (1.76 MB)

Tarih

2017

Yazarlar

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Elsevier Sci Ltd

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Gum Arabic (GA) microgels were successfully prepared via reverse micellization method with high yield (78.5 +/- 5.0%) in 5-100 mu m size range using divinyl sulfone (DVS) as a crosslinker. The GA microgels were degraded hydrolytically 22.8 +/- 3.5% at pH 1 in 20 days, whereas no degradation was observed at pH 7.4 and pH 9 at 37 degrees C. By using diethylenetriamine (DETA), and taurine (TA) as chemical modifying agents, GA microgels were chemically modified as GA-DETA and GA-TA, and the zeta potential values of 5.2 +/- 4.1 and -24.8 +/- 1.3 mV were measured, respectively in comparison to -27.3 +/- 4.2 mV for GA. Moreover, blood compatibility of GA, GA-TA, and GA-DETA microgels was tested via in vitro protein adsorption, % hemolysis ratio, and blood clotting index. All the microgels were hemocompatible with% hemolysis ratio between 0.23 to 2.05, and the GA microgels were found to be highly compatible with a blood clotting index of 81 +/- 40. The biocompatibility of GA, GA-DETA and GA-Taurine microgels against L929 fibroblast cells also revealed 84.4, 89.1, and 67.0% cell viability, respectively, at 25.0 mu g/mL concentration, suggesting great potential in vivo biomedical applications up to this concentration. In addition, 5 and 10 mgImL minimum inhibition concentrations of protonated GA-DETA microgels (GA-DETA-HCl) were determined against E. coli and S. aureus, respectively. (C) 2016 Elsevier Ltd. All rights reserved.

Açıklama

Sagbas Suner, Selin/0000-0002-3524-0675; Sahiner, Nurettin/0000-0003-0120-530X; Aktas, Nahit/0000-0001-9341-607X

Anahtar Kelimeler

Gum Arabic microgels/nanogels, Modifiable GA particle, Degradable/biocompatible GA microgel, Antimicrobial GA microgel

Kaynak

Carbohydrate Polymers

WoS Q DeÄŸeri

Q1

Scopus Q DeÄŸeri

Q1

Cilt

156

Sayı

Künye

closedAccess

Bağlantı

https://doi.org/10.1016/j.carbpol.2016.09.052
 https://hdl.handle.net/20.500.12587/7047

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