Higher alkyl sulfatase activity required by microbial inhabitants to remove anionic surfactants in the contaminated surface waters

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dc.contributor.authorIcgen, Bulent
dc.contributor.authorSalik, Salih Batuhan
dc.contributor.authorGoksu, Lale
dc.contributor.authorUlusoy, Huseyin
dc.contributor.authorYilmaz, Fadime
dc.date.accessioned2020-06-25T18:22:38Z
dc.date.available2020-06-25T18:22:38Z
dc.date.issued2017
dc.departmentKırıkkale Üniversitesi
dc.descriptionIcgen, Bulent/0000-0001-8114-4230
dc.description.abstractBiodegradation of anionic surfactants, like sodium dodecyl sulfate (SDS) are challenged by some bacteria through the function of the enzyme alkyl sulfatases. Therefore, identifying and characterizing bacteria capable of degrading SDS with high alkyl sulfatase enzyme activity are pivotal. In this study, bacteria isolated from surfactant contaminated river water were screened for their potential to degrade SDS. Primary screening carried out by the conventional enrichment culture technique and assessment of SDS-degrading ability through methylene blue active substance assay revealed 12, out of 290, SDS-degrading surface water bacteria with maximum SDS degrading abilities of 46-94% in 24-54 h. The isolates exhibited optimum growth at SDS concentration of 1 g/L, but tolerated up to 15-75 g/L. Eleven isolates were identified as the species of Pseudomonas and one isolate was identified as Aeromonas through 16S rRNA sequencing. Proteolytic activity of alkyl sulfatases in the identified isolates was shown by using native-PAGE analysis. The determined enzyme activities changed in between 1.32 and 2.90 U/mg in the crude extracts. Preliminary experiments showed that the isolates with the alkyl sulfatase enzyme activities >= 2.50 U/mg were strong gratuitous degraders. However, their relative importance in soil, sewage, and wastewater treatment plants remains to be assessed.en_US
dc.description.sponsorshipMiddle East Technical University Research Fun [BAP-03-11-2015-003]en_US
dc.description.sponsorshipThe authors would like to thank Middle East Technical University Research Fund Project for their financial support through the project BAP-03-11-2015-003.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.2166/wst.2017.402
dc.identifier.endpage2366en_US
dc.identifier.issn0273-1223
dc.identifier.issn1996-9732
dc.identifier.issue9en_US
dc.identifier.pmid29144294
dc.identifier.scopus2-s2.0-85034653736
dc.identifier.scopusqualityQ2
dc.identifier.startpage2357en_US
dc.identifier.urihttps://doi.org/10.2166/wst.2017.402
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6843
dc.identifier.volume76en_US
dc.identifier.wosWOS:000415638000011
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherIwa Publishingen_US
dc.relation.ispartofWater Science And Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectanionic surfactantsen_US
dc.subjectSDSen_US
dc.subjectsurfactant contaminationen_US
dc.subjectsurfactant degradationen_US
dc.titleHigher alkyl sulfatase activity required by microbial inhabitants to remove anionic surfactants in the contaminated surface watersen_US
dc.typeArticle

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