Cytotoxicity Testing of Temporary Luting Cements with Two- and Three-Dimensional Cultures of Bovine Dental Pulp-Derived Cells

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dc.contributor.authorUlker, Hayriye Esra
dc.contributor.authorUlker, Mustafa
dc.contributor.authorGumus, Hasan Onder
dc.contributor.authorYalcin, Muhammet
dc.contributor.authorSengun, Abdulkadir
dc.date.accessioned2020-06-25T18:07:40Z
dc.date.available2020-06-25T18:07:40Z
dc.date.issued2013
dc.departmentKırıkkale Üniversitesi
dc.descriptionSENGUN, Abdulkadir/0000-0002-6053-160X
dc.description.abstractThis study evaluated the cytotoxicity of eugenol-containing and eugenol-free temporary luting cements. For cytotoxicity testing, bovine pulp-derived cells transfected with Simian virus 40 Large T antigen were exposed to extracts of eugenol-containing (Rely X Temp E) and eugenol-free (Provicol, PreVISION CEM, and Rely X Temp NE) temporary luting cements for 24 h. The cytotoxicity of the same materials was also evaluated in a dentin barrier test device using three-dimensional cell cultures of bovine pulp-derived cells. The results of the cytotoxicity studies with two-dimensional cultures of bovine dental pulp-derived cells revealed that cell survival with the extracts of Rely X Temp E, Provicol, PreVISION CEM, and Rely X Temp NE was 89.1%, 84.9%, 92.3%, and 66.8%, respectively. Rely X Temp NE and Provicol showed cytotoxic effects on bovine dental pulp-derived cells (P < 0.05). The results of the dentin barrier test revealed that cell survival with the above-mentioned temporary cement was 101.5%, 91.9%, 93.5%, and 90.6%, respectively. None of the temporary luting cements significantly reduced cell survival compared with the negative control in the dentin barrier test (P > 0.05). Biologically active materials released from temporary luting cements may not influence the dentine-pulp complex if the residual dentine layer is at least 0.5 mm thick.en_US
dc.identifier.citationHayriye Esra Ülker, Mustafa Ülker, Hasan Önder Gümüş, Muhammet Yalçın, Abdulkadir Şengün, "Cytotoxicity Testing of Temporary Luting Cements with Two- and Three-Dimensional Cultures of Bovine Dental Pulp-Derived Cells", BioMed Research International, vol. 2013, Article ID 910459, 6 pages, 2013.en_US
dc.identifier.doi10.1155/2013/910459
dc.identifier.issn2314-6133
dc.identifier.pmid23984419
dc.identifier.scopus2-s2.0-84883166573
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1155/2013/910459
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5635
dc.identifier.wosWOS:000323072000001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherHindawi Publishing Corporationen_US
dc.relation.ispartofBiomed Research International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleCytotoxicity Testing of Temporary Luting Cements with Two- and Three-Dimensional Cultures of Bovine Dental Pulp-Derived Cellsen_US
dc.typeArticle

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