Effect of picroside II on erythrocyte deformability and lipid peroxidation in rats subjected to hind limb ischemia reperfusion injury

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dc.contributor.authorComu, Faruk Metin
dc.contributor.authorKilic, Yigit
dc.contributor.authorOzer, Abdullah
dc.contributor.authorKirisci, Mehmet
dc.contributor.authorDursun, Ali Dogan
dc.contributor.authorTatar, Tolga
dc.contributor.authorArslan, Mustafa
dc.date.accessioned2020-06-25T18:22:29Z
dc.date.available2020-06-25T18:22:29Z
dc.date.issued2016
dc.departmentKırıkkale Üniversitesi
dc.descriptionArslan, Mustafa/0000-0003-4882-5063; KARTAL, HAKAN/0000-0003-4539-0228; DURSUN, ALI DOGAN/0000-0001-9056-0025
dc.description.abstractBackground: Ischemia reperfusion injury (I/R) in hind limb is a frequent and important clinical phenomenon. Many structural and functional damages are observed in cells and tissues in these kinds of injuries. In this study, we aimed to evaluate the effect of picroside II on lipid peroxidation and erythrocyte deformability during I/R in rats. Methods: Rats were randomly divided into four groups-each containing six animals (sham, I/R, sham + picroside II, and I/R + picroside II). The infrarenal section of the abdominal aorta was occluded with an atraumatic microvascular clamp in I/R groups. The clamp was removed after 120 minutes and reperfusion was provided for a further 120 minutes. Picroside II (10 mg.kg(-1)) was administered intraperitoneally to the animals in the appropriate groups (sham + picroside II, I/R + picroside II groups). All rats were euthanized by intraperitoneal administration of ketamine (100 mg.kg(-1)) and taking blood from the abdominal aorta. Erythrocytes were extracted from heparinized complete blood samples. Buffer (PT) and then erythrocytes (PE) were passed through the filtration system and the changes in pressure were measured to investigate the role of serum malondialdehyde and nitric oxide (NO) in lipid peroxidation and erythrocyte deformability index. Results: Deformability index was significantly increased in the I/R group compared to groups sham, sham + picroside-II, and I/R + picroside-II (P<0.0001, P<0.0001, and P=0.007). Malondialdehyde (MDA) and NO levels were evaluated. MDA level and NO activity were also higher in the I/R group than in the other groups. Picroside II treatment before hind limb I/R prevented these changes. Conclusion: These results support that deformability of erythrocytes is decreased in I/R injury and picroside II plays a critical role to prevent these alterations. Further experimental and clinical studies are needed to evaluate and clarify the molecular mechanisms of action and clinical importance of these findings.en_US
dc.identifier.citationÇomu, F. M., Kılıç, Y., Özer, A., Kirişçi, M., Dursun, A. D., Tatar, T., Zor, M. H., Kartal, H., Küçük, A., Boyunağa, H., & Arslan, M. (2016). Effect of picroside II on erythrocyte deformability and lipid peroxidation in rats subjected to hind limb ischemia reperfusion injury. Drug design, development and therapy, 10, 927–931.en_US
dc.identifier.endpage931en_US
dc.identifier.issn1177-8881
dc.identifier.pmid27041996
dc.identifier.scopus2-s2.0-84959491065
dc.identifier.scopusqualityQ1
dc.identifier.startpage927en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6763
dc.identifier.volume10en_US
dc.identifier.wosWOS:000371020800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherDove Medical Press Ltden_US
dc.relation.ispartofDrug Design Development And Therapy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjecthind limb ischemia reperfusion injuryen_US
dc.subjectlipid peroxidationen_US
dc.subjectmalondialdehydeen_US
dc.subjectnitric oxideen_US
dc.subjecterythrocyte deformabilityen_US
dc.subjectpicroside IIen_US
dc.titleEffect of picroside II on erythrocyte deformability and lipid peroxidation in rats subjected to hind limb ischemia reperfusion injuryen_US
dc.typeArticle

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