Association between GSTP1 gene polymorphism and serum alpha-GST concentrations undergoing sevoflurane anaesthesia

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dc.contributor.authorKaymak, C.
dc.contributor.authorKarahalil, B.
dc.contributor.authorOzcan, N. N.
dc.contributor.authorOztuna, D.
dc.date.accessioned2020-06-25T17:44:52Z
dc.date.available2020-06-25T17:44:52Z
dc.date.issued2008
dc.description.abstractBackground and objectives: The measurement of alpha-glutathione-S-transferase enzyme Is one of the most sensitive indicators of hepatocellular function. Variation in the glutathione-S-transferase PI gene clusters has been intensively investigated and polymorphism has been described. The aim of the study was to assess whether an association exists between glutathione-S-transferase P1 gene polymorphism and serum alpha-glutathione-S-transferase concentrations for the first postoperative day in patients who underwent anaesthesia with sevoflurane. Methods: In all, 54 unrelated patients were enrolled in this study. Anaesthesia was induced with thiopental and fentanyl. Vecuronium was used for neuromuscular relaxation before endotracheal intubation. Anaesthesia was maintained with sevoflurane in a gas mixture containing 50% nitrous oxide in oxygen. Peripheral venous blood samples to determine serum alpha-glutathione-S-transferase concentrations were collected before induction (T-1), at the end of anaesthesia (T-2) and at 24-h postoperatively (T-3). Enzyme-linked immunosorbent assay (ELISA) immunoassay was used to measure alpha-glutathione-S-transferase levels. Genomic DNA was isolated from serum samples using a genomic DNA purification kit. In order to detect the variants of glutathione-S-transferase P1, polymerase chain reaction - restriction fragment length polymorphism analysis was employed. Results: Early postoperative serum alpha-glutathione-S-transferase levels for all patients were significantly increased when compared with preanaesthetic and 24-h postoperatively (P < 0.05). Serum alpha-glutathione-S-transferase concentrations, in individuals with glutathione-S-transferase P1 Ile105Val genotypes (heterozygote gene), remained elevated at 24 h (P < 0.05), whereas levels in individuals with glutathione-S-transferase P1 Ile105Ile (wild gene) decreased (P > 0.05). Conclusions: Although alpha-glutathione-S-transferase levels were elevated in all patients after sevoflurane anaesthesia, levels remained high at 24 h in patients with glutathione-S-transferase P1 Ile105Val genotypes compared to controls.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1017/S0265021507002724
dc.identifier.endpage199en_US
dc.identifier.issn0265-0215
dc.identifier.issn1365-2346
dc.identifier.issue3en_US
dc.identifier.pmid17892615
dc.identifier.scopus2-s2.0-38649118896
dc.identifier.scopusqualityQ1
dc.identifier.startpage193en_US
dc.identifier.urihttps://doi.org10.1017/S0265021507002724
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4214
dc.identifier.volume25en_US
dc.identifier.wosWOS:000253802900004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherLippincott Williams & Wilkinsen_US
dc.relation.ispartofEuropean Journal Of Anaesthesiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectglutathione S-transferaseen_US
dc.subjectGSTP1 polymorphismen_US
dc.subjectanaesthesia inhalationalen_US
dc.subjectsevofluraneen_US
dc.titleAssociation between GSTP1 gene polymorphism and serum alpha-GST concentrations undergoing sevoflurane anaesthesiaen_US
dc.typeArticle

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