Fibronectin purification from human plasma in a packed-bed column system with gelatin immobilized PHEMA microspheres

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dc.contributor.authorKayirhan-Denizli, F.
dc.contributor.authorArıca, M.Y.
dc.contributor.authorDenizli, A.
dc.date.accessioned2020-06-25T17:34:54Z
dc.date.available2020-06-25T17:34:54Z
dc.date.issued2001
dc.departmentKırıkkale Üniversitesi
dc.description.abstractBioaffinity chromatography has a unique and powerful role that is used as a purification tool in the production of therapeutic plasma protein derivatives. In this study, a bioaffinity-ligand, i.e. gelatin, was covalently immobilized with PHEMA microspheres (150-200 tm in diameter). The affinity sorbent carrying 7.5 mg gelatin g(-1) polymer was then used to separate fibronectin from human plasma in a packed-bed column system. Fibronectin separation from human plasma on unmodified PHEMA microspheres was 0.45 mg g(-1), while much higher adsorption values, up to 21.8 mg g(-1), were obtained with gelatin-immobilized microspheres. The fibronectin adsorption capacity of the microspheres decreased with an increase in the recirculation rate of plasma. Fibronectin adsorption increased with decreasing temperature, and the maximum adsorption achieved at 4 degreesC (26.3 mg fibronecting(-1)). Up to 94.7% of the adsorbed fibronectin was desorbed by using 2 M urea in the presence of I Ni sodium chloride as elution agent. The adsorption-desorption cycle was repeated ten times using the same affinity column. There was no remarkable reduction in the adsorption capacity of the gelatin-immobilized PHEMA microspheres.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1163/156856201300194225
dc.identifier.endpage489en_US
dc.identifier.issn0920-5063
dc.identifier.issue5en_US
dc.identifier.pmid11469779
dc.identifier.scopus2-s2.0-0034947263
dc.identifier.scopusqualityQ1
dc.identifier.startpage479en_US
dc.identifier.urihttps://doi.org/10.1163/156856201300194225
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2945
dc.identifier.volume12en_US
dc.identifier.wosWOS:000171991800002
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherVsp Bven_US
dc.relation.ispartofJournal Of Biomaterials Science-Polymer Edition
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectfibronectin isolationen_US
dc.subjecthuman plasmaen_US
dc.subjectpacked-bed columnen_US
dc.subjectaffinity sorbenten_US
dc.subjectgelatinen_US
dc.subjectPHEMA microspheresen_US
dc.titleFibronectin purification from human plasma in a packed-bed column system with gelatin immobilized PHEMA microspheresen_US
dc.typeArticle

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