Potential c-myc Antisense Oligonucleotide Carriers: PCl/PEG/PEI and PLL/PEG/PEI
Yükleniyor...
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Informa Healthcare
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this work, positively charged, micelle-forming polymers were synthesized and used as a model vector to deliver antisense oligodeoxynucleotide (ASODN) into melanoma cells. Polymers and polymer/ASODN complexes were characterized by DLS according to size, charge, and critical micelle concentration. Nanosize and spherical complexes were observed by AFM. Complexes did not reveal significant toxicity to melanoma cells. Antiproliferative effect of the complexes was observed by immunocytochemical staining and estimated as 56.8% with N/P:9. High amount of apoptosis and very small amount of necrosis were estimated. According to the results, these positively charged polymers forming micelle-like structures seem promising as ASODN carriers.
Açıklama
Dincer, Sevil/0000-0002-6887-6549
Anahtar Kelimeler
antisense oligonucleotide, non-viral vectors, c-myc, poly(lactide), poly(caprolactone), poly(ethylene glycol), polyethylenemine, micelle
Kaynak
Artificial Cells Blood Substitutes And Biotechnology
WoS Q Değeri
Q3
Scopus Q Değeri
N/A
Cilt
39
Sayı
3
Künye
closedAccess
