The Effect of Whole Gut Irrigation on Contractile Responses of Guinea Pig Gallbladder, Ileum, and Tissue Cholecystokinin Levels

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dc.contributor.authorTurkmen, Feyza
dc.contributor.authorSoyer, Tutku
dc.contributor.authorKeskil, Zuhal
dc.contributor.authorKisa, Ucler
dc.contributor.authorAslan, Mustafa
dc.contributor.authorSenyucel, Mine
dc.contributor.authorCakmak, Murat
dc.date.accessioned2020-06-25T18:12:15Z
dc.date.available2020-06-25T18:12:15Z
dc.date.issued2014
dc.departmentKırıkkale Üniversitesi
dc.descriptionKISA, Ucler/0000-0002-8131-6810; Soyer, Tutku/0000-0003-1505-6042
dc.description.abstractAim An experimental study was performed to evaluate the effect of whole gut irrigation (WGI) solutions on contractile responses of the gallbladder and ileum and also on tissue cholecystokinin (CCK) levels. Materials and Methods Thirty guinea pigs were enrolled into five groups including control group (CG) and WGI group (saline physiologic [SP], Ringer lactated [RL], polyethylene glycol [PEG], and dibasic sodium phosphate [DNP]). After median laparotomy, the distal esophagus was ligated and SP, PEG, RL, and DNP infusions (2 mL/kg/min) were performed via gastric catheter until rectal discharge became clear in WGI groups. Ileum and gallbladder samples were obtained for in vitro and biochemical studies without irrigation in CG and after irrigation with different WGI solutions. Isolated ileum and gallbladder preparations were suspended in organ baths for contractile responses of carbachol and CCK. Also, biochemical analysis of tissue CCK levels was performed in ileum and gallbladder samples. Results In PEG group, gallbladder and ileum CCK levels were significantly higher than CG (p < 0.05). Also, DNP irrigation caused increased CCK levels in gallbladder samples (p < 0.05). In lower carbachol concentrations, PEG group showed increased contraction responses in gallbladder samples when compared with controls (p < 0.05). However, ileal responses to carbachol did not show any significant difference between groups, contraction responses to CCK was decreased in PEG group when compared with CG (p < 0.05). Conclusion Among WGI solutions, PEG caused the highest CCK levels in gallbladder and ileum samples. Different WGI solutions affected the contractile responses of gallbladder and ileum smooth muscles divergently. Increased levels of CCK in PEG group support the decreased contractile responses in ileum. Therefore, our results confirm that the effect of WGI on gallbladder and ileum contractility may be CCK related.en_US
dc.description.sponsorshipKirikkale University Scientific Research CouncilKirikkale Universityen_US
dc.description.sponsorshipThis study is supported by Kirikkale University Scientific Research Council and presented at the 28th National Congress of Turkish Association of Pediatric Surgeons, September 22-25, 2010, Antalya, Turkey. Special thanks to Serkan Sahinoglu, MS4 for his kind contribution to review the English of the manuscript.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1055/s-0033-1337115
dc.identifier.endpage173en_US
dc.identifier.issn0939-7248
dc.identifier.issn1439-359X
dc.identifier.issue2en_US
dc.identifier.pmid23460465
dc.identifier.scopus2-s2.0-84897954270
dc.identifier.scopusqualityQ1
dc.identifier.startpage168en_US
dc.identifier.urihttps://doi.org/10.1055/s-0033-1337115
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5831
dc.identifier.volume24en_US
dc.identifier.wosWOS:000337346800008
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherGeorg Thieme Verlag Kgen_US
dc.relation.ispartofEuropean Journal Of Pediatric Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectwhole gut irrigationen_US
dc.subjectgallbladderen_US
dc.subjectcholecystokininen_US
dc.titleThe Effect of Whole Gut Irrigation on Contractile Responses of Guinea Pig Gallbladder, Ileum, and Tissue Cholecystokinin Levelsen_US
dc.typeArticle

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