MLST genotypes and quinolone resistance profiles of Campylobacter jejuni isolates from various sources in Turkey

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dc.authoridHIZLISOY, Harun/0000-0003-3391-0185
dc.authoridKarakaya, Emre/0000-0003-2390-6190
dc.contributor.authorAydin, Fuat
dc.contributor.authorKayman, Tuba
dc.contributor.authorAbay, Secil
dc.contributor.authorHizlisoy, Harun
dc.contributor.authorSaticioglu, Izzet Burcin
dc.contributor.authorKarakaya, Emre
dc.contributor.authorSahin, Orhan
dc.date.accessioned2025-01-21T16:42:44Z
dc.date.available2025-01-21T16:42:44Z
dc.date.issued2023
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThis study was conducted to determine the overall genetic diversity, as well as prevalence and mechanisms of resistance to quinolone antibiotics of 178 Campylobacter jejuni isolated from humans, cattle, dogs, and chickens in Turkey. Multilocus sequence typing (MLST) and E-test were performed for genotyping and antimicrobial susceptibility testing, respectively. Mismatch Amplification Mutation Assay, Polymerase Chain Reaction (MAMAPCR) was used to detect point mutations associated with quinolone resistance. Of the 178 isolates tested, 151 were included in 21 clonal complexes (CCs); the remaining 27 isolates did not belong to any existing CCs. CC21, CC353, CC206, and CC257 were the predominant clones, representing 38 % of all C. jejuni isolates tested. The isolates were assigned to 78 different sequence types (STs), three of which were novel (ST 8082, ST 8083, and ST 8084). Resistance to quinolones was found in 73 (41 %) of the isolates (42.85 %, 2.85 %, 20.58 %, and 43.75 % in human, cattle, dog, and chicken isolates, respectively). All of the resistant isolates had Thr-86-Ile mutation in the gyrA gene. The highest Sorensen coefficient index was detected for human/chicken meat and human/dog C. jejuni isolates (Ss = 0.71), suggesting a strong link between the isolates from respective sources. The Simpson diversity index of C. jejuni isolates analyzed was detected between 0.92 and 0.98.The study provides detailed information on the quinolone resistance and MLST-based genetic relatedness of C. jejuni isolates from humans, cattle, dog, and broiler meat in Turkey for the first time, enabling a better understanding of the transmission pathways of C. jejuni in this country. Our results suggest that broiler meat and dogs may be the most important sources of human campylobacteriosis in Turkey.
dc.description.sponsorshipScientific Research Council of Erciyes University, Kayseri, Turkey [TSA-2013-4275]
dc.description.sponsorshipFunding The funding was provided by the Scientific Research Council of Erciyes University, Kayseri, Turkey (Project No: TSA-2013-4275) .
dc.identifier.doi10.1016/j.ijfoodmicro.2023.110137
dc.identifier.issn0168-1605
dc.identifier.issn1879-3460
dc.identifier.pmid36842255
dc.identifier.scopus2-s2.0-85148766646
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.ijfoodmicro.2023.110137
dc.identifier.urihttps://hdl.handle.net/20.500.12587/25115
dc.identifier.volume391
dc.identifier.wosWOS:000946636500001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofInternational Journal of Food Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectC; jejuni; Multilocus sequence typing (MLST); Quinolone resistance
dc.titleMLST genotypes and quinolone resistance profiles of Campylobacter jejuni isolates from various sources in Turkey
dc.typeArticle

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