Preparation and characterisation of surfaces properties of poly(hydroxyethylmethacrylate-co-methacrylolyamido-histidine) membranes: application for purification of human immunoglobulin G

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dc.contributor.authorArica, M.Y.
dc.contributor.authorYalcin, E.
dc.contributor.authorBayramoglu, G.
dc.date.accessioned2020-06-25T17:40:04Z
dc.date.available2020-06-25T17:40:04Z
dc.date.issued2004
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIn this study, an affinity membrane containing L-histidine as an amino acid ligand was used in separation and purification of human immunoglobulin G (HIgG) from solution and human serum. The polarities and the surface free energies of the affinity membranes were determined by contact angle measurements. HIgG adsorption and purification onto the affinity membranes from aqueous solution and human serum were investigated in a batch and a continuous system. Effect of different system parameters such as ligand density, adsorbent dosage, pH, temperature, ionic strength and HIgG initial concentration on HIgG adsorption were investigated. The maximum adsorption capacity of p(HEMA-MAAH-4) membranes for HIgG was 13.06 mg ml(-1). The reversible HIgG adsorption on the affinity membrane obeyed both the Langmuir and Freundlich isotherm models. The adsorption data was analysed using the first- and second-order kinetic model and the experimental data was well described by the first-order equations. In the continuous system, the purity of the eluted HIgG, as determined by HPLC, was 93% with recovery 58% for p(HEMA-MAAH-4) membrane. The affinity membranes are stable when subjected to sanitization with sodium hydroxide after repeated adsorption-elution cycles. (C) 2004 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.jchromb.2004.04.025
dc.identifier.endpage325en_US
dc.identifier.issn1570-0232
dc.identifier.issn1873-376X
dc.identifier.issue2en_US
dc.identifier.pmid15203046
dc.identifier.scopus2-s2.0-2942627782
dc.identifier.scopusqualityQ2
dc.identifier.startpage315en_US
dc.identifier.urihttps://doi.org/10.1016/j.jchromb.2004.04.025
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3249
dc.identifier.volume807en_US
dc.identifier.wosWOS:000222283800021
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevieren_US
dc.relation.ispartofJournal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectpoly(hydroxyethylmethacrylate-co-en_US
dc.subjectmethacrylolyamido-histidine)en_US
dc.subjectimmunoglobulin Gen_US
dc.titlePreparation and characterisation of surfaces properties of poly(hydroxyethylmethacrylate-co-methacrylolyamido-histidine) membranes: application for purification of human immunoglobulin Gen_US
dc.typeArticle

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