Genoprotective role of vitamin E and selenium in rabbits anaesthetized with sevoflurane

Basit Öğe Kaydı
dc.contributor.authorKaymak, C
dc.contributor.authorKadioglu, E
dc.contributor.authorBasar, H
dc.contributor.authorSardas, S
dc.date.accessioned2020-06-25T17:40:05Z
dc.date.available2020-06-25T17:40:05Z
dc.date.issued2004
dc.departmentKırıkkale Üniversitesi
dc.descriptionSardas, Semra/0000-0001-5456-8636
dc.description.abstractIn this study, genotoxic effects of repeated sevoflurane anaesthesia were investigated in rabbits with or without antioxidant supplementation. Twenty-one New Zealand male rabbits were included in the study and randomized into three groups as: placebo treated ( Group I), vitamin E supplemented ( Group II) and selenium supplemented ( Group III). Vitamin E and selenium were given intraperitoneally for 15 days before anaesthesia treatment. Anaesthesia was administered using 3% sevoflurane in 4 L/min oxygen for a 3-hour period and continued for 3 days. Blood samples were collected before anaesthesia ( Sample 1), after the first, second and third days of sevoflurane administration ( Sample 2, Sample 3 and Sample 4 respectively) and the last samples were taken 5 days after the last sevoflurane administration ( Sample 5). Genotoxic damage was examined using the comet assay. The degree of damage is assessed by grading the cells into three categories of no migration (NM), low migration (LM) and high migration (HM) depending on the fraction of DNA pulled out into the tail under the influence of the electric field. The number of comets in each sample was calculated ( 1 x number of comets in category NM + 2 x number of comets in category LM + 3 x number of comets in category HM) and expressed as the total comet score (TCS), which summarizes the damage frequencies. In Group I, a significant increase in the mean TCSs was observed for Samples 3 and 4 as compared with Sample 1. However, there were no significant differences between Samples 1, 2 and 5. The mean TCS of Sample 4 was significantly higher than Sample 1, 2 and 3 in Group II. Group III demonstrated no significant mean TCSs for any experimental conditions. Statistical differences were also observed between the groups with significant P values. This experimental study points out the presence of DNA damage with repeated sevoflurane anaesthesia and the genoprotective role of antioxidant supplementation on DNA damage in mononuclear leukocytes of rabbits by highly sensitive comet assay.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1191/0960327104ht463oa
dc.identifier.endpage419en_US
dc.identifier.issn0960-3271
dc.identifier.issn1477-0903
dc.identifier.issue8en_US
dc.identifier.pmid15346723
dc.identifier.scopus2-s2.0-4444234926
dc.identifier.scopusqualityQ2
dc.identifier.startpage413en_US
dc.identifier.urihttps://doi.org/10.1191/0960327104ht463oa
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3258
dc.identifier.volume23en_US
dc.identifier.wosWOS:000223054200006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSage Publications Ltden_US
dc.relation.ispartofHuman & Experimental Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectantioxidantsen_US
dc.subjectcomet assayen_US
dc.subjectDNA damageen_US
dc.subjectsevofluraneen_US
dc.titleGenoprotective role of vitamin E and selenium in rabbits anaesthetized with sevofluraneen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Genoprotective role of vitamin E and selenium in rabbits anaesthetized with sevoflurane.pdf
Boyut:
1.26 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu