New metal chelate sorbent for albumin adsorption: Cibacron blue F3GA-Zn(II) attached microporous poly(HEMA) membranes
Yükleniyor...
Tarih
1998
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
John Wiley & Sons Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Poly(2-hydroxyethyl methacrylate) [poly(HEMA)] membranes were prepared by UV-initiated photopolymerization of HEMA in the presence of an initiator (alpha-alpha'-azobis-isobutyronitrile, AIBN). The triazine dye Cibacron Blue F3GA was attached as an affinity ligand to poly(HEMA) membranes, covalently. These affinity membranes with a swelling ratio of 58% and containing 10.7 mmol Cibacron Blue F3GA/m(2) were used in the albumin adsorption studies. After dye-attachment, Zn(TI) ions were chelated within the membranes via attached-dye molecules. Different amounts of Zn(II) ions [650-1440 mg Zn(II)/m(2)] were loaded on the membranes by changing the initial concentration of Zn(II) ions and pH. Bovine serum albumin (BSA) adsorption on these membranes from aqueous solutions containing different amounts of BSA at different pH was investigated in batch reactors. The nonspecific adsorption of BSA on the poly(HEMA) membranes was negligible. Cibacron Blue F3GA attachment significantly increased the BSA adsorption up to 92.1 mg BSA/m(2). Adsorption capacity was further increased when Zn(II) ions were attached (up to 114.8 mg BSA m(2)). More than 90% of the adsorbed BSA was desorbed in 1 h in the desorption medium containing 0.5M NaSCN at pH 8.0 and 0.025M EDTA at pH 4.9. (C) 1998 John Wiley & Sons, Inc.
Açıklama
Anahtar Kelimeler
albumin adsorption, affinity membranes, poly(HEMA), metal chelate adsorption, Cibacron Blue F3GA
Kaynak
Journal Of Applied Polymer Science
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
68
Sayı
4
Künye
closedAccess
