New metal chelate sorbent for albumin adsorption: Cibacron blue F3GA-Zn(II) attached microporous poly(HEMA) membranes

dc.contributor.authorDenizli, A
dc.contributor.authorSalih, B
dc.contributor.authorSenel, S
dc.contributor.authorArica, MY
dc.date.accessioned2020-06-25T17:34:37Z
dc.date.available2020-06-25T17:34:37Z
dc.date.issued1998
dc.departmentKırıkkale Üniversitesi
dc.description.abstractPoly(2-hydroxyethyl methacrylate) [poly(HEMA)] membranes were prepared by UV-initiated photopolymerization of HEMA in the presence of an initiator (alpha-alpha'-azobis-isobutyronitrile, AIBN). The triazine dye Cibacron Blue F3GA was attached as an affinity ligand to poly(HEMA) membranes, covalently. These affinity membranes with a swelling ratio of 58% and containing 10.7 mmol Cibacron Blue F3GA/m(2) were used in the albumin adsorption studies. After dye-attachment, Zn(TI) ions were chelated within the membranes via attached-dye molecules. Different amounts of Zn(II) ions [650-1440 mg Zn(II)/m(2)] were loaded on the membranes by changing the initial concentration of Zn(II) ions and pH. Bovine serum albumin (BSA) adsorption on these membranes from aqueous solutions containing different amounts of BSA at different pH was investigated in batch reactors. The nonspecific adsorption of BSA on the poly(HEMA) membranes was negligible. Cibacron Blue F3GA attachment significantly increased the BSA adsorption up to 92.1 mg BSA/m(2). Adsorption capacity was further increased when Zn(II) ions were attached (up to 114.8 mg BSA m(2)). More than 90% of the adsorbed BSA was desorbed in 1 h in the desorption medium containing 0.5M NaSCN at pH 8.0 and 0.025M EDTA at pH 4.9. (C) 1998 John Wiley & Sons, Inc.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/(SICI)1097-4628(19980425)68:4<657
dc.identifier.endpage664en_US
dc.identifier.issn0021-8995
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-0032565251
dc.identifier.scopusqualityQ2
dc.identifier.startpage657en_US
dc.identifier.urihttps://doi.org/10.1002/(SICI)1097-4628(19980425)68:4<657
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2803
dc.identifier.volume68en_US
dc.identifier.wosWOS:000072741500017
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJohn Wiley & Sons Incen_US
dc.relation.ispartofJournal Of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectalbumin adsorptionen_US
dc.subjectaffinity membranesen_US
dc.subjectpoly(HEMA)en_US
dc.subjectmetal chelate adsorptionen_US
dc.subjectCibacron Blue F3GAen_US
dc.titleNew metal chelate sorbent for albumin adsorption: Cibacron blue F3GA-Zn(II) attached microporous poly(HEMA) membranesen_US
dc.typeArticle

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