The canonical Brucella species-host dependency is changing, however, the antibiotic susceptibility profiles remain unchanged

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dc.authoridErsoy, Yaren/0000-0002-8004-3398
dc.authoridCoskun, Mustafa Reha/0000-0002-1441-3995
dc.authoridBALKAN BOZLAK, CIGDEM EDA/0000-0003-3922-7758
dc.authoridAkar, Mustafa/0000-0002-5589-2849
dc.authoridKarakaya, Emre/0000-0003-2390-6190
dc.contributor.authorCelik, Elif
dc.contributor.authorKayman, Tuba
dc.contributor.authorBuyuk, Fatih
dc.contributor.authorSaglam, Aliye Gulmez
dc.contributor.authorAbay, Secil
dc.contributor.authorAkar, Mustafa
dc.contributor.authorKarakaya, Emre
dc.date.accessioned2025-01-21T16:44:54Z
dc.date.available2025-01-21T16:44:54Z
dc.date.issued2023
dc.departmentKırıkkale Üniversitesi
dc.description.abstractBrucellosis is a chronic disease caused by Brucella species with a wide range of hosts, from marine mammals to terrestrial species, but with strict host preferences. With the zoonotic character, the prevalence of human brucellosis cases is a reflection of animal infections. This study aimed to identify 192 Brucella isolates obtained from various sources by Bruce-ladder PCR and to determine their antibiotic susceptibilities by gradient diffusion method (E-test). As a result of the PCR, all human isolates (n = 57) were identified as B. melitensis. While 58 (82.9%) of the cattle isolates were identified as B. abortus, 59 (90.8%) of the sheep isolates were identified as B. melitensis. In addition, 12 (17.1%) of the cattle isolates and 6 (9.2%) of the sheep isolates were determined as B. melitensis and B. abortus, respectively. The primary host change behavior of B. melitensis was 1.9 times higher than that of B. abortus. While gentamicin and ciprofloxacin susceptibilities of Brucella isolates were 100%, tetracycline, doxycycline, streptomycin, trimethoprim/sulfamethoxazole and rifampicin susceptibilities were 99%, 99%, 97.4%, 91.7% and 83.9%, respectively. The lowest sensitivity of the isolates was determined against to cefoperazone as 26%. A triple-drug resistance was detected in 1 B. abortus isolate that included simultaneous resistance to cefoperazone, rifampicin, and trimethoprim/sulfamethoxazole. The high susceptibility profiles we found against to antibiotics such as tetracycline, doxycycline gentamicin and ciprofloxacin, used widely in treatment, are encouraging. However, the change in the canonical Brucella species-primary host preference suggests the need to reconsider eradication program, including updating vaccine formulations.
dc.identifier.doi10.1016/j.micpath.2023.106261
dc.identifier.issn0882-4010
dc.identifier.issn1096-1208
dc.identifier.pmid37488036
dc.identifier.scopus2-s2.0-85165695712
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.micpath.2023.106261
dc.identifier.urihttps://hdl.handle.net/20.500.12587/25545
dc.identifier.volume182
dc.identifier.wosWOS:001049614900001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Ltd- Elsevier Science Ltd
dc.relation.ispartofMicrobial Pathogenesis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectBruce-ladder PCR; Canonical Brucella species; Cattle; E -test; Human; Sheep
dc.titleThe canonical Brucella species-host dependency is changing, however, the antibiotic susceptibility profiles remain unchanged
dc.typeArticle

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