Assessment of electromechanically stimulated bone marrow stem cells seeded acellular cardiac patch in a rat myocardial infarct model

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dc.authoridGültekinoğlu, Merve/0000-0002-1021-1915
dc.authoridZeybek, Dilara/0000-0002-6161-5661
dc.authoridAksoy, Eda Ayse/0000-0003-4977-0412
dc.authoridÖztürk, Şükrü/0000-0001-9684-634X
dc.contributor.authorÖztürk, Şükrü
dc.contributor.authorShahbazi, Reza
dc.contributor.authorZeybek, Naciye Dilara
dc.contributor.authorKurum, Baris
dc.contributor.authorGültekinoğlu, Merve
dc.contributor.authorAksoy, Eda Ayse
dc.contributor.authorDemircin, Metin
dc.date.accessioned2025-01-21T16:36:00Z
dc.date.available2025-01-21T16:36:00Z
dc.date.issued2021
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIn this study, we evaluated cardiomyogenic differentiation of electromechanically stimulated rat bone marrow-derived stem cells (rt-BMSCs) on an acellular bovine pericardium (aBP) and we looked at the functioning of this engineered patch in a rat myocardial infarct (MI) model. aBP was prepared using a detergent-based decellularization procedure followed by rt-BMSCs seeding, and electrical, mechanical, or electromechanical stimulations (3 millisecond pulses of 5 V cm(-1) at 1 Hz, 5% stretching) to enhance cardiomyogenic differentiation. Furthermore, the electromechanically stimulated patch was applied to the MI region over 3 weeks. After this period, the retrieved patch and infarct region were evaluated for the presence of calcification, inflammatory reaction (CD68), patch to host tissue cell migration, and structural sarcomere protein expressions. In conjunction with any sign of calcification, a higher number of BrdU-labelled cells, and a low level of CD68 positive cells were observed in the infarct region under electromechanically stimulated conditions compared with static conditions. More importantly, MHC, SAC, Troponin T, and N-cad positive cells were observed in both infarct region, and retrieved engineered patch after 3 weeks. In a clear alignment with other results, our developed acellular patch promoted the expression of cardiomyogenic differentiation factors under electromechanical stimulation. Our engineered patch showed a successful integration with the host tissue followed by the cell migration to the infarct region.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TuBTAK) [213M685]
dc.description.sponsorshipThis study was supported by Scientific and Technological Research Council of Turkey (TuBTAK), Grant Number: 213M685. We thank to Professor Dr. Gamze Torun Kose and her research group members for helping for flow cytometry analysis.
dc.identifier.doi10.1088/1748-605X/ac199a
dc.identifier.issn1748-6041
dc.identifier.issn1748-605X
dc.identifier.issue5
dc.identifier.pmid34330118
dc.identifier.scopus2-s2.0-85114443893
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1088/1748-605X/ac199a
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24231
dc.identifier.volume16
dc.identifier.wosWOS:000685759800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherIop Publishing Ltd
dc.relation.ispartofBiomedical Materials
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.subjectcardiac tissue engineering; rt-BMSCs; acellular bovine pericardium; electromechanical stimulation; myocardial infarct model
dc.titleAssessment of electromechanically stimulated bone marrow stem cells seeded acellular cardiac patch in a rat myocardial infarct model
dc.typeArticle

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