Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using real-time cell analysis

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dc.contributor.authorMalkoc, Meral Arslan
dc.contributor.authorDemir, Necla
dc.contributor.authorSengun, Abdulkadir
dc.contributor.authorBozkurt, Serife Buket
dc.contributor.authorHakki, Sema Sezgin
dc.date.accessioned2020-06-25T18:15:43Z
dc.date.available2020-06-25T18:15:43Z
dc.date.issued2015
dc.departmentKırıkkale Üniversitesi
dc.descriptionSENGUN, Abdulkadir/0000-0002-6053-160X
dc.description.abstractPURPOSE. To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs). MATERIALS AND METHODS. Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 mu L of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments. RESULTS. All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P<.001). When the cells were exposed to media by Ultratemp, the cell viability was similar to that of the control at 24 hours (P>.05); however, the cell viability was significantly reduced at 72 hours (P<.001). Light and scanning electron microscopy examination confirmed these results. CONCLUSION. The cytotoxic effects of temporary cements on pulpal tissue should be evaluated when choosing cement for luting provisional restorations.en_US
dc.identifier.citationMalkoç MA, Demİr N, Şengün A, Bozkurt ŞB, Hakki SS. Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using realtime cell analysis. J Adv Prosthodont. 2015 Feb;7(1):21-26.en_US
dc.identifier.doi10.4047/jap.2015.7.1.21
dc.identifier.endpage26en_US
dc.identifier.issn2005-7806
dc.identifier.issn2005-7814
dc.identifier.issue1en_US
dc.identifier.pmid25722833
dc.identifier.scopus2-s2.0-84923002917
dc.identifier.scopusqualityQ1
dc.identifier.startpage21en_US
dc.identifier.urihttps://doi.org/10.4047/jap.2015.7.1.21
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6231
dc.identifier.volume7en_US
dc.identifier.wosWOS:000350184300004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherKorean Acad Prosthodonticsen_US
dc.relation.ispartofJournal Of Advanced Prosthodontics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCytotoxicityen_US
dc.subjectTemporary cementsen_US
dc.subjectReal-time cell analysisen_US
dc.subjectDental pulp-derived cellsen_US
dc.titleCytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using real-time cell analysisen_US
dc.typeArticle

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