Covalent immobilization of α-amylase onto pHEMA microspheres: preparation and application to fixed bed reactor

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dc.contributor.authorARICA, MY
dc.contributor.authorHASIRCI, V
dc.contributor.authorALAEDDINOGLU, NG
dc.date.accessioned2020-06-25T17:34:33Z
dc.date.available2020-06-25T17:34:33Z
dc.date.issued1995
dc.departmentKırıkkale Üniversitesi
dc.description.abstractMicrospheres of poly(2-hydroxyethyl methacrylate) with and without cross-linker were prepared by suspension polymerization. As the amount of cross-linker increased, the equilibrium water content, enzyme loading, immobilization efficiency and recovered activity were all adversely affected. Enzyme alpha-amylase was immobilized onto the microspheres after activation with epichlorohydrin. The K-m value for the immobilized enzyme (0.90% w/v) was much greater than that of the free enzyme (0.53% w/v). It was found that the inactivation constant (ki) increased from 2.23 x 10(-8) min(-1) at 20 degrees C to 1.45 x 10(-4) min(-1) at 60 degrees C. Since the enzyme activity increased as the temperature increased, the temperature profile yielded a peak at 50 degrees C. For free enzyme this is at 45 degrees C. The residence time was proportional to the percentage hydrolysis until a residence time of 12 min was reached. Beyond this the activity increase could not match the increase in residence time. The pH profile yielded a broadening upon immobilization in addition to a small shift to higher pH (from 5.5 to 6.0). The continuous run at 30 degrees C, 1.0% w/v starch concentration and flow rate of 40 cm(3) h(-1) led to only 20% loss in activity after a 120 h operation.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/0142-9612(95)99638-3
dc.identifier.endpage768en_US
dc.identifier.issn0142-9612
dc.identifier.issue10en_US
dc.identifier.pmid7492706
dc.identifier.scopus2-s2.0-0029329146
dc.identifier.scopusqualityQ1
dc.identifier.startpage761en_US
dc.identifier.urihttps://doi.org/10.1016/0142-9612(95)99638-3
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2763
dc.identifier.volume16en_US
dc.identifier.wosWOS:A1995RE49800003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofBiomaterials
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPolymerizationen_US
dc.subjectPHEMA microspheresen_US
dc.subjectenzyme immobilizationen_US
dc.subjectα-amylaseen_US
dc.subjectenzyme reactoren_US
dc.titleCovalent immobilization of α-amylase onto pHEMA microspheres: preparation and application to fixed bed reactoren_US
dc.typeArticle

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