A screening for the occurrence of autoreactive antibodies in sera of pregnant and non-pregnant bitches

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dc.contributor.authorSchafer-Somi, S.
dc.contributor.authorHerkner, K. R.
dc.contributor.authorNeubauer, S.
dc.contributor.authorEgerbacher, M.
dc.contributor.authorPatzl, M.
dc.contributor.authorPollak, S
dc.contributor.authorAslan, S.
dc.date.accessioned2020-06-25T17:41:06Z
dc.date.available2020-06-25T17:41:06Z
dc.date.issued2006
dc.departmentKırıkkale Üniversitesi
dc.descriptionFINDIK, Murat/0000-0003-1408-2548;
dc.description.abstractSera of healthy pregnant (group I, n = 11) and non-pregnant (group II, n = 11) bitches were screened for autoantibodies (AAb). In both groups, blood samples were drawn every fifth day between days 5 and 55 after mating. Serum was analysed via indirect immunofluorescence (IIF) with the Canine ANA HEp-2 Screening Kit((R)). In all animals, anticytoplasmic AAb were detected. Utilizing primate-heart substrate slides AAb against contractile proteins of the cytoplasm could be observed. The predominating fluorescence pattern in pregnant animals resembled above all desmin, which was proven via Western blot. The sera were then pre-incubated with tropomyosin, actin, vimentin, vinculin and keratin solutions, and assessed on HEp-2 slides and on human and canine fibroblasts as well. The latter substrate was used to verify whether the detected Ab were in fact AAb. Utilizing tropomyosin, revealed elimination of the cytoplasmic fluorescences on all three substrates. It is therefore assumed, that in sera of healthy dogs, AAb against contractile structure proteins of the cytoplasm are present regularly. The majority of pregnant bitches presented with higher end-point titres (EPT), than to be found in non-pregnant dogs. AAb against desmin played the key role in those patterns. In addition, sera were screened for thyroid specific AAb, namely thyroglobulin, thyroid peroxidase (TPO), T-3 and T-4, and for AAb against insulin by ELISA or Western blot (TPO). Only in two of the pregnant bitches a weak positive reaction (1 : 100) for T-3-AAb was detected.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1111/j.1439-0531.2006.00636.x
dc.identifier.endpage54en_US
dc.identifier.issn0936-6768
dc.identifier.issn1439-0531
dc.identifier.issue1en_US
dc.identifier.pmid16420328
dc.identifier.scopus2-s2.0-33645108416
dc.identifier.scopusqualityQ2
dc.identifier.startpage48en_US
dc.identifier.urihttps://doi.org/10.1111/j.1439-0531.2006.00636.x
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3631
dc.identifier.volume41en_US
dc.identifier.wosWOS:000234650200009
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWileyen_US
dc.relation.ispartofReproduction In Domestic Animals
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleA screening for the occurrence of autoreactive antibodies in sera of pregnant and non-pregnant bitchesen_US
dc.typeArticle

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