Silver nanoparticles: cytotoxic, apoptotic, and necrotic effects on MCF-7 cells

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dc.contributor.authorCiftci, Hakan
dc.contributor.authorTurk, Mustafa
dc.contributor.authorTamer, Ugur
dc.contributor.authorKarahan, Siyami
dc.contributor.authorMenemen, Yusuf
dc.date.accessioned2020-06-25T18:07:38Z
dc.date.available2020-06-25T18:07:38Z
dc.date.issued2013
dc.departmentKırıkkale Üniversitesi
dc.descriptionTamer, Ugur/0000-0001-9989-6123
dc.description.abstractThe present study was conducted to examine cytotoxic, apoptotic, and necrotic effects of silver nanoparticles (AgNPs) on MCF-7 cells. Colloidal AgNPs were fabricated in an alkaline pH environment via reduction of silver nitrate with hydroxylamine hydrochloride. The size of AgNPs was measured by atomic force microscopy (AFM), transmission electron microscopy (TEM), and dynamic light scattering. Zeta potential of AgNPs was determined by laser Doppler microelectrophoresis. After exposing MCF-7 cells to AgNPs for 24 h, cytotoxicity was measured by WST-1 assay. Apoptosis and necrosis in MCF-7 cells were detected by Annexin-V-FLUOS immunostaining and double staining of Hoechst dye with propidium iodide. In AFM and TEM analyses, the sizes of AgNPs varied from 16 nm to 20 nm. AgNPs were 80 nm in hydrodynamic diameter with a zeta potential of -38.2 mV. The WST-1 assay resulted in an IC50 value of 40 mu g/mL. AgNPs caused apoptotic and necrotic effects in a dose-dependent manner The apoptotic effect of AgNPs was marked up to a concentration of 80 mu g/mL AgNPs. At higher concentrations, the apoptotic effect decreased while the necrotic effect became prominent. The results indicate that AgNPs with a zeta potential of -38.2 mV and hydrodynamic diameter of 80 nm can be used in vitro at concentrations of up to 40 mu g/mL.en_US
dc.identifier.citationÇiftçi H., Türk M., Tamer U., Karahan S., Menemen Y. (2013). Silver nanoparticles: cytotoxic, apoptotic, and necrotic effects on MCF-7 cells. Turkish Journal of Biology, 37(5), 573 - 581.en_US
dc.identifier.doi10.3906/biy-1302-21
dc.identifier.endpage581en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-84883517033
dc.identifier.scopusqualityQ1
dc.identifier.startpage573en_US
dc.identifier.trdizinid150061
dc.identifier.urihttps://doi.org/10.3906/biy-1302-21
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5627
dc.identifier.volume37en_US
dc.identifier.wosWOS:000325300500009
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSilveren_US
dc.subjectnanopartideen_US
dc.subjectMCF-7en_US
dc.subjectcanceren_US
dc.subjectapoptosisen_US
dc.subjectnecrosisen_US
dc.subjectcytotoxicityen_US
dc.titleSilver nanoparticles: cytotoxic, apoptotic, and necrotic effects on MCF-7 cellsen_US
dc.typeArticle

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