Concanavalin a immobilized affinity adsorbents for reversible use in yeast invertase adsorption

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dc.contributor.authorYavuz, Handan
dc.contributor.authorAkgöl, Sinan
dc.contributor.authorArıca, Yakup
dc.contributor.authorDenizli, Adil
dc.date.accessioned2020-06-25T17:40:06Z
dc.date.available2020-06-25T17:40:06Z
dc.date.issued2004
dc.departmentKırıkkale Üniversitesi
dc.descriptionAkgol, Sinan/0000-0002-8528-1854; AKGOL, Sinan/0000-0003-2836-7181
dc.description.abstractConcanavalin A (Con A) immobilized poly(2-hydroxyethyl methacrylate) (PHEMA) beads were investigated for specific adsorption of yeast invertase from aqueous solutions. PHEMA beads were prepared by a suspension polymerization technique with an average size of 150-200 mum, and activated by epichlorohydrin. Con A was then immobilized by covalent binding onto these beads. The maximum Con A immobilization was found to be 10 mg/g. The invertase-loading capability of the PHEMA/Con A beads was observed at pH 5.0. The values of the Michaelis constant K-m of invertase were significanly larger upon adsorption, indicating decreased smaller for the adsorbed invertase. Adsorption improved the pH stability of the enzyme activity was found to be quite stable in repeated experiments.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/mabi.200400028
dc.identifier.endpage679en_US
dc.identifier.issn1616-5187
dc.identifier.issn1616-5195
dc.identifier.issue7en_US
dc.identifier.pmid15468261
dc.identifier.scopus2-s2.0-4544283885
dc.identifier.scopusqualityQ1
dc.identifier.startpage674en_US
dc.identifier.urihttps://doi.org/10.1002/mabi.200400028
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3268
dc.identifier.volume4en_US
dc.identifier.wosWOS:000222909600006
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofMacromolecular Bioscience
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectconcanavalin Aen_US
dc.subjectenzyme immobilizationen_US
dc.subjectglycoenzymeen_US
dc.subjectimmobilizationen_US
dc.subjectinvertaseen_US
dc.subjectsucrose hydrolysisen_US
dc.titleConcanavalin a immobilized affinity adsorbents for reversible use in yeast invertase adsorptionen_US
dc.typeArticle

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