Plasmid-Mediated Colistin Resistance in Escherichia coli O157:H7 Cattle and Sheep Isolates and Whole-Genome Sequence of a Colistin-Resistant Sorbitol Fermentative Escherichia coli O157:H7

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dc.contributor.authorAyaz, Naim Deniz
dc.contributor.authorCufaoglu, Gizem
dc.contributor.authorYonsul, Yesim
dc.contributor.authorGoncuoglu, Muammer
dc.contributor.authorErol, Irfan
dc.date.accessioned2020-06-25T18:34:00Z
dc.date.available2020-06-25T18:34:00Z
dc.date.issued2019
dc.departmentKırıkkale Üniversitesi
dc.descriptionGoncuoglu, Muammer/0000-0001-7245-1941
dc.description.abstractThe aims of this study were to investigate the plasmid-mediated colistin resistance genes (mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5), phenotypic colistin resistance in Escherichia coli O157:H7(+)/H7(-) strains isolated from cattle and sheep, and whole-genome sequence (WGS) analysis of colistin-resistant sorbitol fermentative E. coli O157:H7. According to the results, 5 of the 49 isolates were found to harbor mcr-2 and/or mcr-3 genes. Three isolates, including a sorbitol fermentative E. coli O157:H7, were found phenotypically resistant to colistin with a minimum inhibitory concentration value of 128 mu g/mL. The genome of sorbitol fermentative E. coli O157:H7 did not show 100% similarity to any of the other genome sequences found in the universal genome database. It has also been determined that this isolate carried 62 different antimicrobial resistance genes. This is the first report of plasmid-mediated mcr-2 and mcr-3 genes carrying E. coli O157:H7 from cattle and sheep isolates and WGS of a colistin-resistant sorbitol fermentative E. coli O157:H7. Findings of this study indicate that cattle and sheep can be an important source of colistin resistance in E. coli O157:H7, and slaughterhouse wastewater might be a significant route for dissemination of the plasmid-mediated colistin genes. Therefore, the use of colistin in veterinary medicine should be restricted to reduce the development of resistance. Also it may be necessary to review the non-sorbitol fermentation-based isolation protocol for not missing the sorbitol fermentative E. coli O157:H7 in epidemiological studies.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1089/mdr.2019.0053
dc.identifier.endpage1506en_US
dc.identifier.issn1076-6294
dc.identifier.issn1931-8448
dc.identifier.issue10en_US
dc.identifier.pmid31314669
dc.identifier.scopus2-s2.0-85076329435
dc.identifier.scopusqualityQ2
dc.identifier.startpage1497en_US
dc.identifier.urihttps://doi.org/10.1089/mdr.2019.0053
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7735
dc.identifier.volume25en_US
dc.identifier.wosWOS:000476250300001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofMicrobial Drug Resistance
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectE.coli O157:H7en_US
dc.subjectcolistinen_US
dc.subjectsorbitol fermentativeen_US
dc.subjectcattleen_US
dc.subjectsheepen_US
dc.titlePlasmid-Mediated Colistin Resistance in Escherichia coli O157:H7 Cattle and Sheep Isolates and Whole-Genome Sequence of a Colistin-Resistant Sorbitol Fermentative Escherichia coli O157:H7en_US
dc.typeArticle

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