The effect of basic fibroblast growth factor and adipose tissue-derived mesenchymal stem cells on wound healing, epithelization and angiogenesis in a tracheal resection and end-to-end anastomosis rat model

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dc.contributor.authorOzpolat, Berkant
dc.contributor.authorGurpinar, Ozer Aylin
dc.contributor.authorAyva, Ebru Sebnem
dc.contributor.authorGazyagci, Serkal
dc.contributor.authorNiyaz, Mehmet
dc.date.accessioned2020-06-25T18:07:00Z
dc.date.available2020-06-25T18:07:00Z
dc.date.issued2013
dc.departmentKırıkkale Üniversitesi
dc.descriptionBayram, Cem/0000-0001-8717-4668; Ozpolat, Berkant/0000-0002-6203-7306; GAZYAGCI, SERKAL/0000-0002-0043-6942
dc.description.abstractBackground: This experimental study aims to investigate whether basic fibroblast growth factor, adipose tissue-derived from mesenchymal stem cells, or a combination of both, has an effect on wound healing, epithelization and angiogenesis in a tracheal resection and end-to-end anastomosis rat model. Methods: During the first phase of the study, mesenchymal stem cells were isolated by the primary explant culture technique from the abdominal adipose tissue of rats. When the cells became confluent, they were passaged and characterized by using immunofluorescence staining technique. The cells were cryopreserved for an in vivo application. The rats were divided into four groups, including: the basic fibroblast growth factor (group 1), the mesenchymal stem cells (group 2), the mesenchymal stem cells and basic fibroblast growth factor (group 3) and control group (group 4). The rats were sacrified at day 60 and the anastomosis was evaluated macroscopically for granulation tissue formation, the stenosis and presence of tracheocutaneous fistula formation, and also microscopically for stenosis, epithelium regeneration, inflammation, collagen formation and neovascularization. Results: The inflammation was significantly lower in the study groups (p=0.004, p=0.014, p=0.004), whereas the collagen formation and epithelial regeneration were significantly higher in the study groups, compared to the control group (p=0.015, p=0.022, p=0.026 and p=0.002, p=0.001, p=0.002). Conclusion: Both basic fibroblast growth factor and adipose tissue-derived mesenchymal stem cells increased epithelial regeneration and connective tissue organization in this rat model. They may be used as an adjuvant therapy to surgical resection in patients undergoing tracheal resection.en_US
dc.description.sponsorshipScientific and Technological Research Council in Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [110S244]en_US
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council in Turkey (TUBITAK) (Project number: 110S244).en_US
dc.identifier.citationÖzpolat B., Gürpınar Ö. A., Ayva E. Ş., Gazyağcı S., Niyaz M. (2013). The effect of basic fibroblast growth factor and adipose tissue-derived mesenchymal stem cells on wound healing, epithelization and angiogenesis in a tracheal resection and end-to-end anastomosis rat model. Türk Göğüs Kalp Damar Cerrahisi Dergisi, 21(4), 1010 - 1019.en_US
dc.identifier.doi10.5606/tgkdc.dergisi.2013.7719
dc.identifier.endpage1019en_US
dc.identifier.issn1301-5680
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84889587683
dc.identifier.scopusqualityQ3
dc.identifier.startpage1010en_US
dc.identifier.trdizinid151567
dc.identifier.urihttps://doi.org/10.5606/tgkdc.dergisi.2013.7719
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5440
dc.identifier.volume21en_US
dc.identifier.wosWOS:000326410100024
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherBaycinar Medical Publ-Baycinar Tibbi Yayinciliken_US
dc.relation.ispartofTurk Gogus Kalp Damar Cerrahisi Dergisi-Turkish Journal Of Thoracic And Cardiovascular Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAdipose tissueen_US
dc.subjectangiogenesisen_US
dc.subjectepithelizationen_US
dc.subjectimmunofluorescence stainingen_US
dc.subjectmesenchymal stein cellen_US
dc.subjecttracheal stenosisen_US
dc.subjectwound healingen_US
dc.titleThe effect of basic fibroblast growth factor and adipose tissue-derived mesenchymal stem cells on wound healing, epithelization and angiogenesis in a tracheal resection and end-to-end anastomosis rat modelen_US
dc.typeArticle

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