Role of NETs in the difference in host susceptibility to Toxoplasma gondii between sheep and cattle

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dc.contributor.authorYıldız, Kader
dc.contributor.authorGökpınar, Sami
dc.contributor.authorGazyağcı, Aycan Nuriye
dc.contributor.authorBabur, Cahit
dc.contributor.authorSursal, Neslihan
dc.contributor.authorAzkur, Ahmet Kürşat
dc.date.accessioned2020-06-25T18:22:53Z
dc.date.available2020-06-25T18:22:53Z
dc.date.issued2017
dc.departmentKırıkkale Üniversitesi
dc.descriptionYILDIZ, Kader/0000-0001-5802-6156; Sursal, Neslihan/0000-0002-4144-9520; Babur, Cahit/0000-0002-6524-3260
dc.description.abstractThe main aim of this study was to compare extracellular traps (NETs) formation by polymorphonuclear neutrophils (PMNs) of cattle and sheep when exposed to T. gondii tachyzoites in vitro. The effects of parasite concentrations and different incubation periods on NETs development in cattle and sheep PMNs were studied. The effect of NET structures on host cell invasion by tachyzoites was also studied. This is the first report of NETs development by sheep and cattle PMNs against T. gondii in vitro. T. gondii-induced extracellular DNA production from PMNs was dependent on tachyzoite concentrations and incubation time in both sheep and cattle. Many nuclear and cytoplasmic changes were observed in sheep and cattle PMNs after exposure to T. gondii tachyzoites. The typical appearance of NETs, with MPO, NE and histone (H3) attached to extracellular DNA, was observed. Tachyzoites were entrapped within this structure. Myeloperoxidase (MPO) activity was higher in the cattle PMNtachyzoite co-cultures than sheep. NETs structures released from sheep PMNs caused mechanical immobilisation of T. gondii tachyzoites, however, NET structures released from cattle PMNs may be lethal to tachyzoites. Bovine MPO may have a lethal effect on T. gondii tachyzoites in vitro during a 3 h incubation. Besides other mechanisms that effect on host susceptibility to T. gondii in sheep and cattle, extracellular traps formation as a part of immunological reactions may be play a role in host susceptibility to T. gondii.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK TOVAG)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [214O288]en_US
dc.description.sponsorshipWe would like to thank Prof. Dr. Serdar Diker from Department of Microbiology of Ankara University Faculty of Veterinary Medicine for his excellent technical support during fluorometric analysis. We are also deeply grateful to Dr. Dogukan Ozen from Biostatistic Department of Ankara University Faculty of Veterinary Medicine for his excellent support in statistical analysis. This work was financially supported by The Scientific and Technological Research Council of Turkey (TUBITAK TOVAG, project no: 214O288). Portion of data was presented at 5th European Immunology & Innate Immunity, Berlin, Germany, July 21-23, 2016.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.vetimm.2017.05.005
dc.identifier.endpage10en_US
dc.identifier.issn0165-2427
dc.identifier.issn1873-2534
dc.identifier.pmid28669381
dc.identifier.scopus2-s2.0-85019957961
dc.identifier.scopusqualityQ1
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.vetimm.2017.05.005
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6944
dc.identifier.volume189en_US
dc.identifier.wosWOS:000406567800001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofVeterinary Immunology And Immunopathology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectToxoplasma gondiien_US
dc.subjectNeutrophilen_US
dc.subjectNetosisen_US
dc.subjectExtracellular trapsen_US
dc.subjectSheepen_US
dc.subjectCattleen_US
dc.subjectIn vitroen_US
dc.titleRole of NETs in the difference in host susceptibility to Toxoplasma gondii between sheep and cattleen_US
dc.typeArticle

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