The comparing of homogenization methods for oxidative and antioxidative analyses of sperm
Yükleniyor...
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Scientific Technical Research Council Turkey-Tubitak
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Pretreatment of sperm for oxidative and antioxidative analysis has been shown to be different in studies. These differences can affect the result of analyses. The aim of this study was to compare the analyses of sperm homogenization methods. The semen of four rams were used in the study. Pretreatment procedures included the rotary, bead, and ultrasonic homogenization methods. Oxidative and antioxidative status of sperm samples was assessed by measuring serum lipid hydroperoxide, total oxidant status, free sulfhydryl groups (SH total thiol), ceruloplasmin, and total antioxidant capacity levels. As a result, the homogenization methods before oxidative and antioxidative status analyses significantly affected the result (P < 0.05), and the ultrasonic homogenization method was the most effective. It is recommended to use the ultrasonic homogenization as a pretreatment method to determine the oxidative and antioxidative status analyses in sperm.
Açıklama
Anahtar Kelimeler
Spermatozoa, oxidative analysis, homogenization, methods
Kaynak
Turkish Journal Of Veterinary & Animal Sciences
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
43
Sayı
6
Künye
Varışlı Ö., Akyol N. (2019). The comparing of homogenization methods for oxidative and antioxidative analyses of sperm. Turkish Journal of Veterinary and Animal Sciences, 43(6), 852 - 856.
