The comparing of homogenization methods for oxidative and antioxidative analyses of sperm

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dc.contributor.authorVarisli, Omer
dc.contributor.authorAkyol, Numan
dc.date.accessioned2020-06-25T18:34:25Z
dc.date.available2020-06-25T18:34:25Z
dc.date.issued2019
dc.departmentKırıkkale Üniversitesi
dc.description.abstractPretreatment of sperm for oxidative and antioxidative analysis has been shown to be different in studies. These differences can affect the result of analyses. The aim of this study was to compare the analyses of sperm homogenization methods. The semen of four rams were used in the study. Pretreatment procedures included the rotary, bead, and ultrasonic homogenization methods. Oxidative and antioxidative status of sperm samples was assessed by measuring serum lipid hydroperoxide, total oxidant status, free sulfhydryl groups (SH total thiol), ceruloplasmin, and total antioxidant capacity levels. As a result, the homogenization methods before oxidative and antioxidative status analyses significantly affected the result (P < 0.05), and the ultrasonic homogenization method was the most effective. It is recommended to use the ultrasonic homogenization as a pretreatment method to determine the oxidative and antioxidative status analyses in sperm.en_US
dc.identifier.citationVarışlı Ö., Akyol N. (2019). The comparing of homogenization methods for oxidative and antioxidative analyses of sperm. Turkish Journal of Veterinary and Animal Sciences, 43(6), 852 - 856.en_US
dc.identifier.doi10.3906/vet-1802-10
dc.identifier.endpage856en_US
dc.identifier.issn1300-0128
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85076226132
dc.identifier.scopusqualityQ3
dc.identifier.startpage852en_US
dc.identifier.trdizinid336147
dc.identifier.urihttps://doi.org/10.3906/vet-1802-10
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7904
dc.identifier.volume43en_US
dc.identifier.wosWOS:000500746600018
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherScientific Technical Research Council Turkey-Tubitaken_US
dc.relation.ispartofTurkish Journal Of Veterinary & Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSpermatozoaen_US
dc.subjectoxidative analysisen_US
dc.subjecthomogenizationen_US
dc.subjectmethodsen_US
dc.titleThe comparing of homogenization methods for oxidative and antioxidative analyses of spermen_US
dc.typeArticle

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