Detection of telomerase activity in bronchial lavage as an adjunct to cytological diagnosis in lung cancer

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dc.contributor.authorDikmen, E.
dc.contributor.authorKara, M.
dc.contributor.authorDikmen, G.
dc.contributor.authorCakmak, H.
dc.contributor.authorDogan, P.
dc.date.accessioned2020-06-25T17:35:36Z
dc.date.available2020-06-25T17:35:36Z
dc.date.issued2003
dc.departmentKırıkkale Üniversitesi
dc.description.abstractObjective: Definitive diagnosis of lung cancer with conventional methods may sometimes be difficult in clinical practice. Telomerase is a ribonucleoprotein DNA polymerase that maintains the telomeric region of chromosomes during successive rounds of cell division. Telomerase activity in body cavity fluids has been advocated to be a potential diagnostic marker for malignancy. We investigated the diagnostic value of telomerase activity in bronchial lavage samples of patients undergoing diagnosis of lung cancer. Methods: A total of 29 bronchial lavage samples were collected from patients in whom the diagnosis was confirmed with cytological and/or histological examinations. Patients were classified as lung cancer patients (Group 1, n = 22) and patients with benign disease (Group 2, n = 7). Telomerase activity was determined with polymerase chain reaction-based TRAP (The telomeric repeat amplification protocol) assay. Results: Cytological examination was diagnostic in 12 (54.5%) of 22 patients in Group 1, and in all seven patients of Group 2 (P = 0.063). Telomerase activity was positive in 16 (72.7%) of Group I patients, while it was positive in only 1 (14.3%) sample of a lung abscess in Group 2 (P = 0.011). The sensitivity rate of cytological examination when combined with telomerase activity (81.8%) was significantly greater than that of cytological examination alone (54.5%) (P = 0.031). The sensitivity and specificity of telomerase activity were 72.7 and 85.7%, respectively. Telomerase activity had a positive predictive value as 0.94 and negative predictive value as 0.50. Diagnostic accuracy of telomerase activity was 75.8%. Conclusion: Telomerase activity in bronchial lavage is a highly sensitive diagnostic biomarker for malignancy and a potential complementary diagnostic technique to cytological examination in the diagnosis of lung cancer. (C) 2002 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.citationDikmen, E., Kara, M., Dikmen, G., Cakmak, H., & Dogan, P. (2003). Detection of telomerase activity in bronchial lavage as an adjunct to cytological diagnosis in lung cancer. European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 23(2),194-199 . https://doi.org/10.1016/s1010-7940(02)00741-8en_US
dc.identifier.doi10.1016/S1010-7940(02)00741-8
dc.identifier.endpage199en_US
dc.identifier.issn1010-7940
dc.identifier.issue2en_US
dc.identifier.pmid12559342
dc.identifier.scopus2-s2.0-0037307836
dc.identifier.scopusqualityQ1
dc.identifier.startpage194en_US
dc.identifier.urihttps://doi.org/10.1016/S1010-7940(02)00741-8
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3160
dc.identifier.volume23en_US
dc.identifier.wosWOS:000180995200013
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofEuropean Journal Of Cardio-Thoracic Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjecttelomeraseen_US
dc.subjectlung canceren_US
dc.subjectbronchial lavageen_US
dc.titleDetection of telomerase activity in bronchial lavage as an adjunct to cytological diagnosis in lung canceren_US
dc.typeArticle

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