The relationship between seropositivity and tissue cysts in sheep naturally infected with Toxoplasma gondii

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dc.contributor.authorYildiz, Kader
dc.contributor.authorKul, Oguz
dc.contributor.authorGokpinar, Sami
dc.contributor.authorAtmaca, Hasan Tarik
dc.contributor.authorGencay, Yilmaz Emre
dc.contributor.authorGazyagci, Aycan Nuriye
dc.contributor.authorGurcan, Ismayil Safa
dc.date.accessioned2020-06-25T18:12:30Z
dc.date.available2020-06-25T18:12:30Z
dc.date.issued2014
dc.departmentKırıkkale Üniversitesi
dc.descriptionGencay, Yilmaz Emre/0000-0002-2154-9663; YILDIZ, Kader/0000-0001-5802-6156; Babur, Cahit/0000-0002-6524-3260; ATMACA, HASAN TARIK/0000-0001-8379-4114
dc.description.abstractSkeletal muscles (tongue, masseter, leg, intercostal, and diaphragmatic muscles) and brain samples of 100 sheep at slaughter were analysed for the presence of T. gondii tissue cysts along with serum IgG titres. Two methods of isolation by percoll gradient centrifugation and tissue microarray (TMA) technique with immunoperoxidase staining were used. Seropositivity was detected in 88% (88/100) of sheep sera analysed by indirect fluorescent antibody test. Tissue cysts were observed in 46 (52.3%, 46/88) and 15 (17%, 15/88) of the seropositive sheep with the isolation technique and TMA and immunoperoxidase staining, respectively. The diameters of the tissue cysts were 25-58 x 25-62 (mean 34 x 36) mu m. The relationship between the presence of tissue cysts and seropositivity in sheep was statistically significant at 1/16 (P < 0.01) and at 1/64 and 1/128 (P < 0.001) serum dilutions.en_US
dc.description.sponsorshipTUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG 110O497]en_US
dc.description.sponsorshipThis work was supported by a grant from TUBITAK (project no: TOVAG 110O497).en_US
dc.identifier.citationYıldız K., Kul O., Gökpınar S., Atmaca H. T., Gencay Y. E., Gazyağcı A. N., Babür K., Gürcan İ. S.(2014). The relationship between seropositivity and tissue cysts in sheep naturally infected with Toxoplasma gondii. Turkish Journal of Veterinary and Animal Sciences, 38(2), 169 - 175.en_US
dc.identifier.doi10.3906/vet-1308-49
dc.identifier.endpage175en_US
dc.identifier.issn1300-0128
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84894524102
dc.identifier.scopusqualityQ3
dc.identifier.startpage169en_US
dc.identifier.trdizinid159337
dc.identifier.urihttps://doi.org/10.3906/vet-1308-49
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5943
dc.identifier.volume38en_US
dc.identifier.wosWOS:000331515700009
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherScientific Technical Research Council Turkey-Tubitaken_US
dc.relation.ispartofTurkish Journal Of Veterinary & Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectToxoplasma gondiien_US
dc.subjecttissue cysten_US
dc.subjectpercoll gradient centrifugationen_US
dc.subjecttissue microarrayen_US
dc.subjectserologyen_US
dc.subjectsheepen_US
dc.subjectTurkeyen_US
dc.titleThe relationship between seropositivity and tissue cysts in sheep naturally infected with Toxoplasma gondiien_US
dc.typeArticle

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