Polymorphisms and Protein Expressions of Glutathione S-Transferase M1 and T1 in Non-Small Cell Lung Cancer

Basit Öğe Kaydı
dc.contributor.authorKilic, Murat
dc.contributor.authorAda, Ahmet Oguz
dc.contributor.authorOguztuzun, Serpil
dc.contributor.authorDemirag, Funda
dc.contributor.authorCelik, Sezgin
dc.contributor.authorBicakcioglu, Pinar
dc.contributor.authorIscan, Mumtaz
dc.date.accessioned2020-06-25T18:22:36Z
dc.date.available2020-06-25T18:22:36Z
dc.date.issued2017
dc.departmentKırıkkale Üniversitesi
dc.descriptionDemirag, Funda/0000-0003-4790-8369; Kilic, Murat/0000-0002-1377-2021; Ada, Ahmet Oguz/0000-0001-9987-0572
dc.description.abstractObjectives: The deletion polymorphisms of glutathione S-transferase (GST) GSTM1 and GSTT1 genes result in the absence of the corresponding protein, which decreases the detoxification of carcinogens. Studies evaluating polymorphisms and protein expressions in the same patients are limited. Therefore, in this study, we aimed to investigate the association between polymorphisms and protein expressions of GSTM1 and GSTT1 in lung tissues of patients with non-small cell lung cancer (NSCLC). Materials and Methods: For protein expression and gene deletion studies, tumor and surrounding tumor free (normal) tissue of 33 patients with NSCLC were used. In paraffin-embedded tissues, immunohistochemistry was used to detect protein expressions, and multiplex polymerase chain reaction amplification was used to identify gene deletions. Results: GSTM1 and GSTT1 protein expressions were not detected in patients with GSTM1 and GSTT1 gene deletions, whereas protein expressions were detected in lung tissues of all patients carrying GSTM1 and GSTT1 genes. The protein expression level of GSTT1 was 2.0-fold higher in tumors of patients lacking GSTM1 genes than those with GSTM1 genes (p=0.018). Protein expression of GSTM1 was statistically higher in tumor tissues than in normal tissues of patients with GSTM1 genes (p=0.001). Conclusion: These results show that a) there is an association between gene deletions and protein expressions of GSTM1 and GSTT1 in patients with NSCLC, b) in the absence of GSTM1 genes, enhancement of expression of GSTT1 in tumors is likely to show that GSTT1 increases its capacity to detoxify the toxic electrophiles in tumors, and c) GSTM1 protein expression is higher in tumors compared with normal lung tissues of patients with NSCLC.en_US
dc.description.sponsorshipKirikkale University, Coordination Unit of Scientific Research Projects [2011/16]en_US
dc.description.sponsorshipThis study was carried out under the project supported by Kirikkale University, Coordination Unit of Scientific Research Projects (Grant number: 2011/16).en_US
dc.identifier.citationKiliç, M., Ada, A. O., Oğuztüzün, S., Demirağ, F., Çelik, S., Biçakçioğlu, P., & Işcan, M. (2017). Polymorphisms and Protein Expressions of Glutathione S-Transferase M1 and T1 in Non-Small Cell Lung Cancer. Turkish journal of pharmaceutical sciences, 14(3), 237–242.en_US
dc.identifier.doi10.4274/tjps.74745
dc.identifier.endpage242en_US
dc.identifier.issn1304-530X
dc.identifier.issue3en_US
dc.identifier.pmid32454619
dc.identifier.scopus2-s2.0-85035013883
dc.identifier.scopusqualityQ2
dc.identifier.startpage237en_US
dc.identifier.urihttps://doi.org/10.4274/tjps.74745
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6823
dc.identifier.volume14en_US
dc.identifier.wosWOS:000417379400004
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Pharmacists Assocen_US
dc.relation.ispartofTurkish Journal Of Pharmaceutical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGSTM1en_US
dc.subjectGSTT1en_US
dc.subjectpolymorphismen_US
dc.subjectprotein expressionen_US
dc.subjectnon-small cell lung carcinomaen_US
dc.titlePolymorphisms and Protein Expressions of Glutathione S-Transferase M1 and T1 in Non-Small Cell Lung Canceren_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Polymorphisms and Protein Expressions of Glutathione S-Transferase M1 and T1 in Non-Small Cell Lung Cancer.pdf
Boyut:
1.2 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu