Stem cell suspension injected HEMA-lactate-dextran cryogels for regeneration of critical sized bone defects

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dc.contributor.authorBolgen, Nimet
dc.contributor.authorKorkusuz, Petek
dc.contributor.authorVargel, Ibrahim
dc.contributor.authorKilic, Emine
dc.contributor.authorGuzel, Elif
dc.contributor.authorCavusoglu, Tarik
dc.contributor.authorPiskin, Erhan
dc.date.accessioned2020-06-25T18:12:21Z
dc.date.available2020-06-25T18:12:21Z
dc.date.issued2014
dc.departmentKırıkkale Üniversitesi
dc.descriptionCetinkaya, Duygu Uckan/0000-0003-3593-6493; Meydanli, E. Elif Guzel/0000-0001-9072-3322; Bayram, Cem/0000-0001-8717-4668; Bolgen, Nimet/0000-0003-3162-0803; Bolgen, Nimet/0000-0003-3162-0803; KORKUSUZ, PETEK/0000-0002-7553-3915; KILIC, Emine/0000-0003-2984-7638
dc.description.abstractHEMA-Lactate-Dextran cryogel scaffolds were produced by cryogelation. Mesencyhmal stem cells (MSC) were isolated from rat bone marrow. Critical sized cranial bone defects were created in rat cranium. Stem cells were injected inside the macropores of the cryogel scaffolds prepared from HEMA-Lactate-Dextran possessing the same dimensions with the defect and placed in the cranial bone. The cryogels placed in the defect without stem cells served as control. After selected time intervals the experimental sites were removed from the animals and new bone formation and tissue integration were investigated by histological analysis. The in vivo results exhibited osseous tissue integration within the implant and mineralized functionally stable bone restoration of the cranial defects. Tissue formation started in the macrospores of the scaffold starting from periphery to the center. A significant ingrowth of connective tissue cells and new blood vessels allowed new bone formation. Histological data demonstrated that new bone per total defect area ratio, were not significantly different in "scaffold-stem cells" group compared to that of "scaffold only" group on all time points. However, the blood vessel density was significantly higher in "scaffold-stem cells" group comparing to that of the "scaffold only" group on day 30. "Scaffold-stem cells" given group gave better tissue response score when compared to "scaffold only" group on day 180.en_US
dc.description.sponsorshipTurkish Academy of Sciences (TUBA)Turkish Academy of Sciencesen_US
dc.description.sponsorshipThis study was performed in the context of the EU-FP6-NoE Expertissues Project. Erhan Piskin was supported by the Turkish Academy of Sciences (TUBA) as a full member.en_US
dc.identifier.citationNimet Bölgen, Petek Korkusuz, İbrahim Vargel, Emine Kılıç, Elif Güzel, Tarık Çavuşoğlu, Duygu Uçkan & Erhan Pişkin (2014) Stem cell suspension injected HEMA-lactate-dextran cryogels for regeneration of critical sized bone defects, Artificial Cells, Nanomedicine, and Biotechnology, 42(1), 70-77.en_US
dc.identifier.doi10.3109/21691401.2013.775578
dc.identifier.endpage77en_US
dc.identifier.issn2169-1401
dc.identifier.issn2169-141X
dc.identifier.issue1en_US
dc.identifier.pmid23477355
dc.identifier.scopus2-s2.0-84892774415
dc.identifier.scopusqualityQ1
dc.identifier.startpage70en_US
dc.identifier.urihttps://doi.org/10.3109/21691401.2013.775578
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5873
dc.identifier.volume42en_US
dc.identifier.wosWOS:000329845100010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofArtificial Cells Nanomedicine And Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectanimal modelen_US
dc.subjectbone tissue engineeringen_US
dc.subjectcranial defecten_US
dc.subjectcryogelsen_US
dc.subjectstem cellsen_US
dc.titleStem cell suspension injected HEMA-lactate-dextran cryogels for regeneration of critical sized bone defectsen_US
dc.typeArticle

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