Evaluation of oxidative stress via protein expression of glutathione S-transferase and cytochrome p450 (CYP450) soenzymes in psoriasis vulgaris patients treated with methotrexate

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dc.contributor.authorAkbulak, Ozge
dc.contributor.authorKaradag, Ayse Serap
dc.contributor.authorAkdeniz, Necmettin
dc.contributor.authorOzkanli, Seyma
dc.contributor.authorOzlu, Emin
dc.contributor.authorZemheri, Ebru
dc.contributor.authorOguztuzun, Serpil
dc.date.accessioned2020-06-25T18:30:07Z
dc.date.available2020-06-25T18:30:07Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIntroduction: Oxidative stress is the imbalance between oxidant-antioxidant systems and may play a major role in the psoriasis pathogenesis. Cytochrome (CYP) is a family of enzymes that are responsible for the metabolism of various endogenous and exogenous substances such as drug metabolism. Most importantly, the antioxidant system is the glutathione S-transferases (GST), which decrease oxidative stress by reducing oxidative products.Aim: We aimed to evaluate the expressions of isoenzymes of GST and CYP families and the beneficial role of metotrexate (MTX) in this process.Material and methods: This study included 21 patients with psoriasis and 22 healthy subjects. We treated all the patients with 10-15mg/week of MTX for minimum 12weeks. Expressions of GST and CYP enzymes were assessed by immunohistochemical staining.Results: GSTK1, GSTM1 and GSTT1 expressions were significantly higher in the psoriasis tissues than in the control tissues (p<0.05; p<0.05; p<0.05, respectively). In the psoriasis patients, GSTO1 expression was similar the control group. CYP1B1 and CYP2E1 expressions were significantly higher in the pre-treatment and post-treatment psoriasis tissues than in the control tissues (p<0.05; p<0.05; p<0.05; p<0.05, respectively).Conclusion: We found a significant increase in the tissue levels of, either expression of GST, or CYP, which has important role in drug metabolism and oxidative stress. MTX treatment resulted in marked clinical improvement, yet we found that MTX did not have any significant effect on these parameters. CYP2E1 is especially the most important enzyme for MTX metabolism since it is the primarily responsible of the toxic metabolism of various drugs. The other experimental studies involving greater number of patients and other different drug are needed to enlighten the role of oxidant and antioxidant systems and the other possible mechanisms for the pathogenesis of psoriasis.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1080/15569527.2017.1369431
dc.identifier.endpage185en_US
dc.identifier.issn1556-9527
dc.identifier.issn1556-9535
dc.identifier.issue2en_US
dc.identifier.pmid28838263
dc.identifier.scopus2-s2.0-85030557801
dc.identifier.scopusqualityQ3
dc.identifier.startpage180en_US
dc.identifier.urihttps://doi.org/10.1080/15569527.2017.1369431
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7564
dc.identifier.volume37en_US
dc.identifier.wosWOS:000429276400012
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofCutaneous And Ocular Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCytochrome P450en_US
dc.subjectglutathione S-transferasesen_US
dc.subjectmethotrexateen_US
dc.subjectpsoriasisen_US
dc.titleEvaluation of oxidative stress via protein expression of glutathione S-transferase and cytochrome p450 (CYP450) soenzymes in psoriasis vulgaris patients treated with methotrexateen_US
dc.typeArticle

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