Primary tumor cells obtained from MNU-induced mammary carcinomas show immune heterogeneity which can be modulated by low-efficiency transfection of CD40L gene
Yükleniyor...
Tarih
2009
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The presence of CD40 on carcinoma cells is an important factor for the generation of tumor-specific responses induced by CD40 ligation. In an N-methyl-N-nitrosourea (MNU)-induced autochthonous mammary tumor model, we analyzed the immune features of primary tumor cells. Here, CD40 was frequently detected on the primary tumor cultures and selectively expressed on the malignant mammary tissue in vivo. On the other hand, every mammary tumor cell culture had a heterogeneous and reduced expression of proinflammatory TNF alpha, IL-1 beta, IL-6 and CXCL1 cytokines compared to normal mammary epithelial cells. Low-efficiency transfection of CD40 ligand (CD40L) gene enhanced the expression of proinflammatory cytokines in the tumor cells, and strengthened allogeneic immune reactions and costimulatory activity which may help overwhelming suppressive features of the tumor.
Açıklama
Esendagli, Gunes/0000-0003-4865-2377;
Anahtar Kelimeler
immune heterogeneity, primary cell culture, mammary cancer, transfection, CD40, CD40 ligand
Kaynak
Cancer Biology & Therapy
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
8
Sayı
2
Künye
Esendagli, G., Canpinar, H., Yilmaz, G., Gunel-Ozcan, A., Oguz Guc, M., Kansu, E., & Guc, D. (2009). Primary tumor cells obtained from MNU-induced mammary carcinomas show immune heterogeneity which can be modulated by low-efficiency transfection of CD40L gene. Cancer Biology & Therapy, 8(2), 136–142. https://doi.org/10.4161/cbt.8.2.7078
