Primary tumor cells obtained from MNU-induced mammary carcinomas show immune heterogeneity which can be modulated by low-efficiency transfection of CD40L gene

Basit Öğe Kaydı
dc.contributor.authorEsendağlı, Günşs
dc.contributor.authorCanpınar, Hande
dc.contributor.authorYılmaz, Güldal
dc.contributor.authorGunel-Ozcan, Aysen
dc.contributor.authorGüç, M. Oğuz
dc.contributor.authorKansu, Emin
dc.contributor.authorGüç, Dicle
dc.date.accessioned2020-06-25T17:48:44Z
dc.date.available2020-06-25T17:48:44Z
dc.date.issued2009
dc.departmentKırıkkale Üniversitesi
dc.descriptionEsendagli, Gunes/0000-0003-4865-2377;
dc.description.abstractThe presence of CD40 on carcinoma cells is an important factor for the generation of tumor-specific responses induced by CD40 ligation. In an N-methyl-N-nitrosourea (MNU)-induced autochthonous mammary tumor model, we analyzed the immune features of primary tumor cells. Here, CD40 was frequently detected on the primary tumor cultures and selectively expressed on the malignant mammary tissue in vivo. On the other hand, every mammary tumor cell culture had a heterogeneous and reduced expression of proinflammatory TNF alpha, IL-1 beta, IL-6 and CXCL1 cytokines compared to normal mammary epithelial cells. Low-efficiency transfection of CD40 ligand (CD40L) gene enhanced the expression of proinflammatory cytokines in the tumor cells, and strengthened allogeneic immune reactions and costimulatory activity which may help overwhelming suppressive features of the tumor.en_US
dc.description.sponsorshipEczacibasi Scientific Research; Hacettepe UniversityHacettepe University [05DO3104001]en_US
dc.description.sponsorshipThis study was supported by Eczacibasi Scientific Research and Award Fund, and Hacettepe University Scientific Research Unit ( project no. 05DO3104001). We wish to thank Dr. Cemaliye Akyerli for her helpful supervision in real time RT-PCR analysis.en_US
dc.identifier.citationEsendagli, G., Canpinar, H., Yilmaz, G., Gunel-Ozcan, A., Oguz Guc, M., Kansu, E., & Guc, D. (2009). Primary tumor cells obtained from MNU-induced mammary carcinomas show immune heterogeneity which can be modulated by low-efficiency transfection of CD40L gene. Cancer Biology & Therapy, 8(2), 136–142. https://doi.org/10.4161/cbt.8.2.7078en_US
dc.identifier.doi10.4161/cbt.8.2.7078
dc.identifier.endpage142en_US
dc.identifier.issn1538-4047
dc.identifier.issn1555-8576
dc.identifier.issue2en_US
dc.identifier.pmid19164932
dc.identifier.scopus2-s2.0-58749106788
dc.identifier.scopusqualityQ1
dc.identifier.startpage136en_US
dc.identifier.urihttps://doi.org/10.4161/cbt.8.2.7078
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4547
dc.identifier.volume8en_US
dc.identifier.wosWOS:000262496900006
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofCancer Biology & Therapy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectimmune heterogeneityen_US
dc.subjectprimary cell cultureen_US
dc.subjectmammary canceren_US
dc.subjecttransfectionen_US
dc.subjectCD40en_US
dc.subjectCD40 liganden_US
dc.titlePrimary tumor cells obtained from MNU-induced mammary carcinomas show immune heterogeneity which can be modulated by low-efficiency transfection of CD40L geneen_US
dc.typeArticle

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