Prevalence and Characterization of Listeria monocytogenes Isolated from Beef and Sheep Carcasses in Turkey with Characterization of Locally Isolated Listeriophages as a Control Measure

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dc.contributor.authorAyaz, Naim Deniz
dc.contributor.authorOnaran, Bahar
dc.contributor.authorCufaoglu, Gizem
dc.contributor.authorGoncuoglu, Muammer
dc.contributor.authorOrmanci, Fatma Seda
dc.contributor.authorErol, Irfan
dc.date.accessioned2020-06-25T18:29:20Z
dc.date.available2020-06-25T18:29:20Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.descriptionGoncuoglu, Muammer/0000-0001-7245-1941; ONARAN, BAHAR/0000-0002-3515-7548
dc.description.abstractSwab samples from cattle and sheep carcasses (120 of each) were tested for Listeria monocytogenes, and 120 slaughterhouse wastewater samples were tested for listeriophages over 12 months (10 samples per month) to note the seasonal distribution. L. monocytogenes and bacteriophage isolates were characterized, and the biocontrol of L. monocytogenes was investigated in meatballs with a phage cocktail. L. monocytogenes was found in 3.4 and 2.5% of cattle and sheep carcasses, respectively. All the isolates were found to harbor hlyA, actA, inlA, inlB, inlC, inlJ, plcA, plcB, fbpA, and fri genes with varied mRNA expression levels by real-time reverse transcriptase PCR analysis. Five isolates did not harbor the vip gene. According to enterobacterial repetitive intergenic consensus PCR, L. monocytogenes isolates were classified into four different groups based on their DNA patterns. The L. monocytogenes isolates were characterized for antibiotic susceptibility; one strain was found to be resistant to five different antibiotic classes. Of 11 lytic listeriophages, two were selected for the cocktail based on their DNA restriction profiles, efficiency of plating, transmission electron microscopy, and in vitro and in vivo analyses. In the biocontrol study, we used a food model that consisted of a novel bacteriophage cocktail in raw meatballs. The highest reduction of L. monocytogenes was recorded as 2.2 log CFU/g at a multiplicity of cellular infection of 4.7 at the end of 1 h. In conclusion, the new bacteriophage cocktail in this study can be considered an efficient biocontrol agent of L. monocytogenes in meatballs.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114R104]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, grant no. 114R104). We thank Prof. Dr. Zekiye Suludere for her technical assistance in obtaining TEM micrographs. Also, we are grateful to Assoc. Prof. Serkan Erat for kindly helping with the statistical analyses.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.4315/0362-028X.JFP-18-310
dc.identifier.endpage2053en_US
dc.identifier.issn0362-028X
dc.identifier.issn1944-9097
dc.identifier.issue12en_US
dc.identifier.pmid30485766
dc.identifier.scopus2-s2.0-85057541678
dc.identifier.scopusqualityQ2
dc.identifier.startpage2045en_US
dc.identifier.urihttps://doi.org/10.4315/0362-028X.JFP-18-310
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7243
dc.identifier.volume81en_US
dc.identifier.wosWOS:000451442400019
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherInt Assoc Food Protectionen_US
dc.relation.ispartofJournal Of Food Protection
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacteriophageen_US
dc.subjectCattleen_US
dc.subjectCharacterizationen_US
dc.subjectListeria monocytogenesen_US
dc.subjectSheepen_US
dc.subjectSlaughterhouse wastewateren_US
dc.titlePrevalence and Characterization of Listeria monocytogenes Isolated from Beef and Sheep Carcasses in Turkey with Characterization of Locally Isolated Listeriophages as a Control Measureen_US
dc.typeArticle

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