Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract

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dc.authoridGUN GOK, Zehra/0000-0001-6426-0395
dc.authoridEkici, Husamettin/0000-0001-6403-737X
dc.authoridEkici, Seda/0000-0002-7982-5261
dc.contributor.authorBozkaya, Ogun
dc.contributor.authorEkici, Husamettin
dc.contributor.authorGun Gok, Zehra
dc.contributor.authorArat, Esra
dc.contributor.authorEkici, Seda
dc.contributor.authorYigitoglu, Mustafa
dc.contributor.authorVargel, Ibrahim
dc.date.accessioned2025-01-21T16:33:23Z
dc.date.available2025-01-21T16:33:23Z
dc.date.issued2023
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIn recent years, researchers have shown an increased interest in using medicinal plants for the synthesis of silver nanoparticles (AgNPs) having various therapeutic properties. Centella asiatica (CA), a medicinal plant, has been used to treat minor burn wounds, psoriasis, and hypertrophic wounds among many other pathological conditions. The current study aimed to synthesize CA coated AgNPs (CA-AgNPs) with appropriate biocompatibility and various therapeutic properties, including antimicrobial and analgesic activities. The synthesized CA-AgNPs were characterized by ultraviolet-visible (UV-Vis) spectroscopy, zeta potential measurements, and fourier transform infrared (FT-IR) spectroscopy. The formation of spherical CA-AgNPs was confirmed by a single surface plasmon resonance (SPR) peak emerging at 420 nm wavelength by UV-Vis. The average hydrodynamic diameter and zeta potential of the particles were found to be 29.5 nm and -24.5 mV, respectively. The FT-IR analyses showed that the AgNPs were coated and stabilized by bioactive compounds from the CA extract. MTT cytotoxicity assay revealed that CA-AgNPs at <= 1 mM concentrations exhibited biocompatibility for L929 fibroblast cells. The antimicrobial activity of CA-AgNPs was confirmed by significant inhibition of Staphylococcus aureus and Escherichia coli. In addition, the analgesic effect of CA-AgNPs was investigated for the first time in the literature by tail-flick and hot plate methods, and statistically significant results were obtained for both methods. Taken together, these results suggest that CA-AgNPs can be used as an effective antibacterial and analgesic agent in a variety of biomedical applications, including coating wound dressings.
dc.description.sponsorshipKirikkale University Scientific Commite [2019/029]; Kirikkale University
dc.description.sponsorshipThis study was financially supported by Kirikkale University Scientific Commite under the project no 2019/029. The authors are grateful to Kirikkale University for their supporting.
dc.identifier.doi10.33988/auvfd.1014802
dc.identifier.endpage96
dc.identifier.issn1300-0861
dc.identifier.issn1308-2817
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85123890079
dc.identifier.scopusqualityQ3
dc.identifier.startpage87
dc.identifier.trdizinid1152645
dc.identifier.urihttps://doi.org/10.33988/auvfd.1014802
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay1152645
dc.identifier.urihttps://hdl.handle.net/20.500.12587/23790
dc.identifier.volume70
dc.identifier.wosWOS:001047216600012
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherAnkara Univ
dc.relation.ispartofAnkara Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.subjectAnalgesic effect; Antibacterial effect; Centella asiatica; Cytotoxicity; Silver nanoparticles
dc.titleInvestigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract
dc.typeArticle

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