Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) reactive membrane utilised for cholesterol oxidase immobilisation
Yükleniyor...
Tarih
2002
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
John Wiley & Sons Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) p(HEMA-GMA) membrane was prepared by UV-initiated photopolymerisation of 2-hydroxyethyl methacrylate (HEMA) and glycidyl methacrylate (GMA) in the presence of an initiator, azobisisobutyronitrile (AIBN). Cholesterol oxidase was immobilised directly on the membrane by forming covalent bonds between its amino groups and the epoxide groups of the membrane. An average of 53 mug of enzyme was immobilised per cm(2) of membrane, and the bound enzyme retained about 67% of its initial activity. Immobilisation improved the pH stability of the enzyme as well as its temperature stability. The optimum temperature was 5degreesC higher than that of the free enzyme and was significantly broader. The thermal inactivation rate constants for free and immobilised preparations at 70degreesC were calculated as k(i (free)) 1.06 x 10(-1) min(-1) and k(i (imm)) 2.68 x 10(-2) min(-1), respectively. The immobilised enzyme activity was found to be quite stable in the repeated experiments. (C) 2002 Society of Chemical Industry.
Açıklama
Akgol, Sinan/0000-0002-8528-1854; AKGOL, Sinan/0000-0003-2836-7181
Anahtar Kelimeler
pHEMA membrane, epoxy support, immobilization, covalent attachment, cholesterol oxidase
Kaynak
Polymer International
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
51
Sayı
12
Künye
closedAccess
