Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) reactive membrane utilised for cholesterol oxidase immobilisation

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dc.contributor.authorAkgöl, S.
dc.contributor.authorBayramoğlu, G.
dc.contributor.authorKaçar, Y.
dc.contributor.authorDenizli, A
dc.contributor.authorArıca, M.Y.
dc.date.accessioned2020-06-25T17:34:56Z
dc.date.available2020-06-25T17:34:56Z
dc.date.issued2002
dc.departmentKırıkkale Üniversitesi
dc.descriptionAkgol, Sinan/0000-0002-8528-1854; AKGOL, Sinan/0000-0003-2836-7181
dc.description.abstractPoly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) p(HEMA-GMA) membrane was prepared by UV-initiated photopolymerisation of 2-hydroxyethyl methacrylate (HEMA) and glycidyl methacrylate (GMA) in the presence of an initiator, azobisisobutyronitrile (AIBN). Cholesterol oxidase was immobilised directly on the membrane by forming covalent bonds between its amino groups and the epoxide groups of the membrane. An average of 53 mug of enzyme was immobilised per cm(2) of membrane, and the bound enzyme retained about 67% of its initial activity. Immobilisation improved the pH stability of the enzyme as well as its temperature stability. The optimum temperature was 5degreesC higher than that of the free enzyme and was significantly broader. The thermal inactivation rate constants for free and immobilised preparations at 70degreesC were calculated as k(i (free)) 1.06 x 10(-1) min(-1) and k(i (imm)) 2.68 x 10(-2) min(-1), respectively. The immobilised enzyme activity was found to be quite stable in the repeated experiments. (C) 2002 Society of Chemical Industry.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/pi.875
dc.identifier.endpage1322en_US
dc.identifier.issn0959-8103
dc.identifier.issue12en_US
dc.identifier.scopus2-s2.0-0346037170
dc.identifier.scopusqualityQ1
dc.identifier.startpage1316en_US
dc.identifier.urihttps://doi.org/10.1002/pi.875
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2959
dc.identifier.volume51en_US
dc.identifier.wosWOS:000180406100004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJohn Wiley & Sons Ltden_US
dc.relation.ispartofPolymer International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectpHEMA membraneen_US
dc.subjectepoxy supporten_US
dc.subjectimmobilizationen_US
dc.subjectcovalent attachmenten_US
dc.subjectcholesterol oxidaseen_US
dc.titlePoly(hydroxyethyl methacrylate-co-glycidyl methacrylate) reactive membrane utilised for cholesterol oxidase immobilisationen_US
dc.typeArticle

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