Differential effects of nitrogen and sulfur deprivation on growth and biodiesel feedstock production of Chlamydomonas reinhardtii

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dc.contributor.authorÇakmak, Turgay
dc.contributor.authorAngun, Pınar
dc.contributor.authorDemiray, Yunus Emre
dc.contributor.authorÖzkan, Alper Devrim
dc.contributor.authorElibol, Zeynep
dc.contributor.authorTekinay, Turgay
dc.date.accessioned2020-06-25T18:06:32Z
dc.date.available2020-06-25T18:06:32Z
dc.date.issued2012
dc.departmentKırıkkale Üniversitesi
dc.descriptionLenka, Sangram K./0000-0002-0121-2430; Elibol Cakmak, Zeynep/0000-0002-6772-5570; Ozkan, Alper/0000-0001-7467-4022
dc.description.abstractBiodiesel production from microalgae is a promising approach for energy production; however, high cost of its process limits the use of microalgal biodiesel. Increasing the levels of triacylglycerol (TAG) levels, which is used as a biodiesel feedstock, in microalgae has been achieved mainly by nitrogen starvation. In this study, we compared effects of sulfur (S) and nitrogen (N) starvation on TAG accumulation and related parameters in wild-type Chlamydomonas reinhardtii CC-124 mt(-) and CC-125 mt(+) strains. Cell division was interrupted, protein and chlorophyll levels rapidly declined while cell volume, total neutral lipid, carotenoid, and carbohydrate content increased in response to nutrient starvation. Cytosolic lipid droplets in microalgae under nutrient starvation were monitored by three-dimensional confocal laser imaging of live cells. Infrared spectroscopy results showed that relative TAG, oligosaccharide and polysaccharide levels increased rapidly in response to nutrient starvation, especially S starvation. Both strains exhibited similar levels of regulation responses under mineral deficiency, however, the degree of their responses were significantly different, which emphasizes the importance of mating type on the physiological response of algae. Neutral lipid, TAG, and carbohydrate levels reached their peak values following 4 days of N or S starvation. Therefore, 4 days of N or S starvation provides an excellent way of increasing TAG content. Although increase in these parameters was followed by a subsequent decline in N-starved strains after 4 days, this decline was not observed in S-starved ones, which shows that S starvation is a better way of increasing TAG production of C. reinhardtii than N starvation. Biotechnol. Bioeng. 2012; 109:19471957. (c) 2012 Wiley Periodicals, Inc.en_US
dc.description.sponsorshipTurkish Ministry of Agriculture and Rural Affairs General Directorate of Agricultural ResearchGida Tarim Ve Hayvancilik Bakanligi [TAGEM-10/AR-GE/26]; State Planning Organization of the Republic of TurkeyTurkiye Cumhuriyeti Kalkinma Bakanligien_US
dc.description.sponsorshipContract grant sponsor: Turkish Ministry of Agriculture and Rural Affairs General Directorate of Agricultural Research; Contract grant number: TAGEM-10/AR-GE/26; Contract grant sponsor: State Planning Organization of the Republic of Turkeyen_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/bit.24474
dc.identifier.endpage1957en_US
dc.identifier.issn0006-3592
dc.identifier.issue8en_US
dc.identifier.pmid22383222
dc.identifier.scopus2-s2.0-84862654930
dc.identifier.scopusqualityQ1
dc.identifier.startpage1947en_US
dc.identifier.urihttps://doi.org/10.1002/bit.24474
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5230
dc.identifier.volume109en_US
dc.identifier.wosWOS:000305451400008
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofBiotechnology And Bioengineering
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbiodieselen_US
dc.subjectChlamydomonas reinhardtiien_US
dc.subjectmicroalgaeen_US
dc.subjectnitrogen starvationen_US
dc.subjectsulfur starvationen_US
dc.subjecttriacylglycerolen_US
dc.titleDifferential effects of nitrogen and sulfur deprivation on growth and biodiesel feedstock production of Chlamydomonas reinhardtiien_US
dc.typeArticle

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