Assessment of oxidative stress and tissue damage in Echinococcus granulosus naturally infected bovine liver

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dc.authoridAlshahrani, Mohammad Y./0009-0006-9288-2523
dc.authoridAlshahrani, Mohammad Y./0009-0005-3873-4565
dc.contributor.authorAnteplioglu, Tugce
dc.contributor.authorYapici, Tilbe Su
dc.contributor.authorDincel, Gungor Cagdas
dc.contributor.authorAl-Olayan, Ebtesam
dc.contributor.authorAlshahrani, Mohammad Y.
dc.contributor.authorEl-Ashram, Saeed
dc.date.accessioned2025-01-21T16:36:01Z
dc.date.available2025-01-21T16:36:01Z
dc.date.issued2024
dc.departmentKırıkkale Üniversitesi
dc.description.abstractEchinococcus granulosus is a zoonotic parasite infects many livestock species, especially cattle, sheep, goat and buffalo, causing cystic echinococcosis. The aim of this study was to demonstrate the presence of the parasite and parasitic tissue damage histopathologically and to determine the role of oxidative stress in the tissue damage through the immunohistochemical detection of the oxidative damage -marker malondialdehyde (MDA) and the antioxidant response -marker superoxide dismutase (SOD). The material of the study consisted of 20 liver samples with Echinococcus cysts and 10 E.granulosus- negative healthy liver samples obtained from different cattle at various times from slaughterhouses in Kirikkale province, Turkey. Histopathologically, Echinococcus cysts of various sizes were observed along with the surrounding fibrous connective tissue. Giant cells, mononuclear cells, and eosinophilic leukocytes were found between the fibrous connective tissue and the cyst. In the parenchymal tissue distant from the cyst, inflammatory changes were observed, including vacuolation and necrosis in hepatocytes, congestion and dilation sinusoidal capillaries. Immunohistochemically, MDA immunopositivity was observed in both hepatocytes surrounding the cyst and areas distant from the cyst, while SOD immunopositivity was mainly detected in fibrous connective tissue and hepatocytes surrounding the Echinococcus cysts. A significant increase in MDA immunoreactivity was observed in E.granulosus s.l.-infected livers. Although no statistically significant change was observed in SOD immunopositivity in the liver tissues with cystic echinococcosis, regional variations were noted. Germinal layer (GL) of Echinococcus cyst showed immunopositive staining for MDA, while laminated layer (LL) exhibited immunonegative staining. To the authors' best understanding, this study represents a pioneering effort in showcasing and evaluating the immunoreactivities of MDA and SOD within the liver tissue afflicted with Echinococcus cysts. Simultaneously, the examination extends to encompass tissue damage and the infiltration of inflammatory cells. This study highlights the role of oxidative stress in the pathogenesis of Cystic Echinococcosis (CE) and the need for further investigation of antioxidant defense mechanisms and their regional variations.
dc.description.sponsorshipKing Saud University in Riyadh, Saudi Arabia [RSP2024R111]
dc.description.sponsorshipAcknowledgements The authors would like to express their gratitude to the Researchers Sup-porting Project (RSP2024R111) at King Saud University in Riyadh, Saudi Arabia.
dc.identifier.doi10.1016/j.tice.2024.102333
dc.identifier.issn0040-8166
dc.identifier.pmid38422584
dc.identifier.scopus2-s2.0-85186751607
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1016/j.tice.2024.102333
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24234
dc.identifier.volume87
dc.identifier.wosWOS:001198966300001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherChurchill Livingstone
dc.relation.ispartofTissue & Cell
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectEchinococcus granulosus; Echinococcus cyst; Liver; MDA; SOD
dc.titleAssessment of oxidative stress and tissue damage in Echinococcus granulosus naturally infected bovine liver
dc.typeArticle

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