Dye-affinity hollow-fibres and their lysozyme adsorption-desorption characteristics
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Date
2001
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
John Wiley & Sons Ltd
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
Dye-affinity adsorption is increasingly used for protein separation. Hollow-fibres have advantages as adsorbents in comparison to conventional bead supports because they are not compressible and can eliminate internal diffusion limitations. The aim of this study was to explore in detail the performance of polyamide hollow-fibres to which Reactive Green HE-4BD was attached for adsorption of lysozyme. The hollow-fibre was characterized by scanning electron microscopy. These dye-carrying hollow-fibres (26.3 mu mol g(-1)) were used in the lysozyme adsorption-elution studies. The effect of initial concentration of lysozyme and medium pH on the adsorption efficiency of dye-attached hollow-fibres was studied in a batch system. The non-specific adsorption of lysozyme on the polyamide hollow-fibres was 1.8 mgg(-1). Reactive Green HE-4BD attachment significantly increased the lysozyme adsorption up to 41.1 mgg(-1). Langmuir adsorption model was found to be applicable in interpreting lead adsorption by Reactive Green HE-4BD attached hollow fibres. Significant amount of the adsorbed lysozyme (up to 95%) was eluted in lh in the elution medium containing 1.0M NaSCN at pH 8.0. In order to determine the effects of adsorption conditions on possible conformational changes of lysozyme structure, fluorescence spectrophotometry was employed. We concluded that polyamide dye-affinity hollow-fibres can be applied for lysozyme adsorption without causing any significant conformational changes. Repeated adsorption-elution processes showed that these dye-attached hollow-fibres are suitable for lysozyme adsorption. (C) 2001 Society of Chemical Industry.
Description
Akgol, Sinan/0000-0002-8528-1854; AKGOL, Sinan/0000-0003-2836-7181
Keywords
hollow-fibre sorbents, Reactive Green HE-4BD, lysozyme, dye-affinity adsorbents, polyamide
Journal or Series
Polymer International
WoS Q Value
Q2
Scopus Q Value
Q1
Volume
50
Issue
10
Citation
closedAccess
