Dye-affinity hollow-fibres and their lysozyme adsorption-desorption characteristics

Basit Öğe Kaydı
dc.contributor.authorŞenel, S.
dc.contributor.authorAkgöl, S.
dc.contributor.authorArica, Y.
dc.contributor.authorDenizli, A.
dc.date.accessioned2020-06-25T17:34:48Z
dc.date.available2020-06-25T17:34:48Z
dc.date.issued2001
dc.departmentKırıkkale Üniversitesi
dc.descriptionAkgol, Sinan/0000-0002-8528-1854; AKGOL, Sinan/0000-0003-2836-7181
dc.description.abstractDye-affinity adsorption is increasingly used for protein separation. Hollow-fibres have advantages as adsorbents in comparison to conventional bead supports because they are not compressible and can eliminate internal diffusion limitations. The aim of this study was to explore in detail the performance of polyamide hollow-fibres to which Reactive Green HE-4BD was attached for adsorption of lysozyme. The hollow-fibre was characterized by scanning electron microscopy. These dye-carrying hollow-fibres (26.3 mu mol g(-1)) were used in the lysozyme adsorption-elution studies. The effect of initial concentration of lysozyme and medium pH on the adsorption efficiency of dye-attached hollow-fibres was studied in a batch system. The non-specific adsorption of lysozyme on the polyamide hollow-fibres was 1.8 mgg(-1). Reactive Green HE-4BD attachment significantly increased the lysozyme adsorption up to 41.1 mgg(-1). Langmuir adsorption model was found to be applicable in interpreting lead adsorption by Reactive Green HE-4BD attached hollow fibres. Significant amount of the adsorbed lysozyme (up to 95%) was eluted in lh in the elution medium containing 1.0M NaSCN at pH 8.0. In order to determine the effects of adsorption conditions on possible conformational changes of lysozyme structure, fluorescence spectrophotometry was employed. We concluded that polyamide dye-affinity hollow-fibres can be applied for lysozyme adsorption without causing any significant conformational changes. Repeated adsorption-elution processes showed that these dye-attached hollow-fibres are suitable for lysozyme adsorption. (C) 2001 Society of Chemical Industry.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/pi.759
dc.identifier.endpage1149en_US
dc.identifier.issn0959-8103
dc.identifier.issue10en_US
dc.identifier.scopus2-s2.0-0035478885
dc.identifier.scopusqualityQ1
dc.identifier.startpage1143en_US
dc.identifier.urihttps://doi.org/10.1002/pi.759
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2897
dc.identifier.volume50en_US
dc.identifier.wosWOS:000171435700013
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJohn Wiley & Sons Ltden_US
dc.relation.ispartofPolymer International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjecthollow-fibre sorbentsen_US
dc.subjectReactive Green HE-4BDen_US
dc.subjectlysozymeen_US
dc.subjectdye-affinity adsorbentsen_US
dc.subjectpolyamideen_US
dc.titleDye-affinity hollow-fibres and their lysozyme adsorption-desorption characteristicsen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Dye-affinity hollow-fibres and their lysozyme adsorption-desorption characteristics.pdf
Boyut:
194.01 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu