Epoxy-derived pHEMA membrane for use bioactive macromolecules immobilization: Covalently bound urease in a continuous model system
Yükleniyor...
Tarih
2000
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
John Wiley & Sons Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Poly(2-hydroxyethylmethacrylate) (pHEMA) membranes were prepared by W-initiated photopolymerization of HEMA in the presence of an initiator (alpha-alpha '-azobisisobutyronitrile, AIBN). The epoxy group, i.e., epichlorohydrin, was incorporated covalently, and the urease was immobilized onto pHEMA membranes by covalent bonding through the epoxy group. The retained activity of the immobilized enzyme was found to be 27%. The K-m values were 18 and 34 mM for the free and the immobilized enzymes, respectively, and the V-max values were found to be 59.7 and 16.2 U mg(-1) for the free and the immobilized enzyme. The optimum pHs was 7.2 for both forms, and the optimum temperature for the free and the immobilized enzymes were determined to be 45 and 50 degrees C, respectively. The immobilized urease was characterized in a continuous system and during urea degradation the operational stability rate constant for the immobilized enzyme was found to be 5.83 x 10(-5) min(-1). (C) 2000 John Wiley & Sons, Inc.
Açıklama
Anahtar Kelimeler
covalent bonding, enzyme immobilization, urease, enzyme reactor, pHEMA membrane
Kaynak
Journal Of Applied Polymer Science
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
77
Sayı
9
Künye
closedAccess
