Epoxy-derived pHEMA membrane for use bioactive macromolecules immobilization: Covalently bound urease in a continuous model system

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dc.contributor.authorArica, MY
dc.date.accessioned2020-06-25T17:34:41Z
dc.date.available2020-06-25T17:34:41Z
dc.date.issued2000
dc.departmentKırıkkale Üniversitesi
dc.description.abstractPoly(2-hydroxyethylmethacrylate) (pHEMA) membranes were prepared by W-initiated photopolymerization of HEMA in the presence of an initiator (alpha-alpha '-azobisisobutyronitrile, AIBN). The epoxy group, i.e., epichlorohydrin, was incorporated covalently, and the urease was immobilized onto pHEMA membranes by covalent bonding through the epoxy group. The retained activity of the immobilized enzyme was found to be 27%. The K-m values were 18 and 34 mM for the free and the immobilized enzymes, respectively, and the V-max values were found to be 59.7 and 16.2 U mg(-1) for the free and the immobilized enzyme. The optimum pHs was 7.2 for both forms, and the optimum temperature for the free and the immobilized enzymes were determined to be 45 and 50 degrees C, respectively. The immobilized urease was characterized in a continuous system and during urea degradation the operational stability rate constant for the immobilized enzyme was found to be 5.83 x 10(-5) min(-1). (C) 2000 John Wiley & Sons, Inc.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/1097-4628(20000829)77:9<2000
dc.identifier.endpage2008en_US
dc.identifier.issn0021-8995
dc.identifier.issue9en_US
dc.identifier.scopus2-s2.0-0034249263
dc.identifier.scopusqualityQ2
dc.identifier.startpage2000en_US
dc.identifier.urihttps://doi.org/10.1002/1097-4628(20000829)77:9<2000
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2846
dc.identifier.volume77en_US
dc.identifier.wosWOS:000087900100016
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJohn Wiley & Sons Incen_US
dc.relation.ispartofJournal Of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcovalent bondingen_US
dc.subjectenzyme immobilizationen_US
dc.subjectureaseen_US
dc.subjectenzyme reactoren_US
dc.subjectpHEMA membraneen_US
dc.titleEpoxy-derived pHEMA membrane for use bioactive macromolecules immobilization: Covalently bound urease in a continuous model systemen_US
dc.typeArticle

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